Specificity of human anti-neurofilament autoantibodies

Journal of Neuroimmunology
D B BraxtonN Latov

Abstract

The specificities and isotypes of human antibodies that react with neurofilament (NF) proteins were examined by Western blot analysis. Two-thirds of the subjects tested had antibodies to the 200 kDa high molecular weight neurofilament protein (NF-H), and fewer had antibodies to the low and middle molecular weight neurofilament proteins (NF-L and NF-M respectively). Human autoantibodies bound to both native and dephosphorylated NF-H, but some antibodies bound to dephosphorylated NF-H only, indicating the presence of at least two target epitopes. They also recognized a fusion protein containing a segment of the NF-H protein produced by a cDNA clone in Escherichia coli, indicating that they bind to unmodified peptide epitopes. The anti-NF-H antibodies were mostly IgG, but were frequently complexed to IgA or IgM antibodies, possibly with rheumatoid factor or anti-idiotypic activity. These characteristics of anti-NF-H antibodies are most consistent with a secondary immune response that is antigen driven and T-cell dependent.

References

May 10, 1979·The New England Journal of Medicine·R K Gershon
Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L GuesdonS Avrameas
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·F C LucaR B Vallee
Apr 1, 1987·The Journal of Cell Biology·W C EarnshawD W Cleveland
Feb 1, 1985·The Journal of Clinical Investigation·R S Schwartz, B D Stollar
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B H TohD Dahl
Apr 11, 1985·Nature·A Lanzavecchia
Jul 23, 1987·The New England Journal of Medicine·S Burdette, R S Schwartz
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L JacobJ F Bach
Oct 1, 1986·The Journal of Experimental Medicine·M W Cunningham, R A Swerlick
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K DellagiD Paulin
Mar 1, 1984·The Journal of Experimental Medicine·A L DeFrancoW E Paul
Mar 16, 1984·Journal of Immunological Methods·P Kurki, I Virtanen
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R S FujinamiH Koprowski
Nov 1, 1983·Analytical Biochemistry·S TokutakeR K Liem
May 1, 1984·Clinical Immunology and Immunopathology·J André-SchwartzR S Schwartz
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L A Sternberger, N H Sternberger
Jun 1, 1983·Journal of the Neurological Sciences·T S ElizanM D Yahr
Mar 1, 1985·Immunology Today·T ManserM L Gefter

❮ Previous
Next ❯

Citations

Jan 1, 1996·Life Sciences·J T PeltolaG K Molnar
Sep 1, 1994·Journal of Neuroimmunology·E Nobile-OrazioG Scarlato
Nov 7, 1994·Neuroscience Letters·F DehautJ F Leterrier
Apr 30, 1998·Journal of the Neurological Sciences·P CouratierM O Jauberteau
Sep 30, 1999·Environmental Health Perspectives·H A El-FawalM Y Shamy
Jan 1, 1990·Journal of Neuroimmunology·J SrinivasanN Latov
Aug 25, 2015·Journal of Neuroimmunology·Jessica A PanzerDavid R Lynch
Apr 21, 2016·Expert Review of Neurotherapeutics·Franz Blaes, Backialakshmi Dharmalingam
Nov 19, 2011·Multiple Sclerosis International·Irena Dujmovic
Nov 1, 2007·Expert Review of Clinical Immunology·Franz BlaesKlaus T Preissner
Sep 19, 2001·Health & Social Care in the Community·Anne P. KennedyDavid Wilkin
Oct 5, 2018·Neurology·Eati BasalAndrew McKeon
Jul 1, 1991·Journal of Neuroscience Research·S A SadiqN Latov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.