PMID: 2112046Mar 1, 1990Paper

Specificity of human natural antibody to recombinant tissue-type plasminogen activator (t-PA) expressed in mouse C127 cells

Chemical & Pharmaceutical Bulletin
J TsujiN Shimizu

Abstract

A natural antibody with binding specificity for recombinant tissue-type plasminogen activator (t-PA) expressed in mouse C127 cells was present in almost all disease-free humans and patients with thrombotic disease examined. This antibody was specific for a carbohydrate, alpha 1-3-linked galactose residue, and was isolated by affinity chromatography using Synsorb 90 coupled with the glycosidic epitope Gal alpha 1-3Gal beta 1-4Glc-R as an immunoadsorbent. The evaluation of various glycoproteins for ability to bind the purified antibody in ELISA demonstrated that not only recombinant t-PA from C127 cells but also recombinant erythropoietin (EPO) and recombinant protein C produced in C127 cells have alpha 1-3-linked galactose residues on their sugar side chains. This anti-alpha-galactosyl antibody also interacted with natural t-PA from human vascular trees (vascular t-PA) and placenta (placenta t-PA), but not to melanoma t-PA, recombinant t-PA, EPO or protein C expressed in Chinese hamster ovary (CHO) cells.

Citations

May 1, 1994·Enzyme and Microbial Technology·N Jenkins, E M Curling
Aug 1, 1996·Nature Biotechnology·N JenkinsD C James
Nov 15, 1993·European Journal of Biochemistry·H Lis, N Sharon
Jun 1, 1995·Baillière's Clinical Haematology·D C Rijken

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