Specificity of inhibition by steroids of porcine oocyte maturation in vitro

The Journal of Experimental Zoology
J D Richter, R W McGaughey

Abstract

In order to examine the influence of several steroids on the process of oocyte maturation, denuded (adherent cumulus granulosa cells mechanically removed) and intact (cumulus granulosa cells left attached) porcine oocytes were cultured in the presence or absence of estradiol-17 beta, estradiol-17 alpha, testosterone, cortisol, progesterone, or the nonsteroidal estrogen diethyl stilbestrol (all at 10 microgram/ml) in defined medium that contained either BSA or dextran. Estradiol-17 beta was the only steroid to exert a significant inhibitory effect on the maturation of denuded oocytes, and did so only in BSA supplemented medium. The inhibition was reversible in that oocytes, cultured in steroid-free medium after initial culture in estradiol-17 beta medium, resumed meiotic maturation. Oocytes took up 3H-estradiol-17 beta in both media, although less radiolabel entered oocytes in BSA supplemented medium. The majority of label in the oocytes, when cultured with either medium, was not displaced by excess radioinert estradiol-17 beta or progesterone, nor were the oocytes saturated even when cultured in 10(-6) M estradiol-17 beta. Autoradiography of sectioned oocytes after culture in 3H-estradiol-17 beta has shown that there was no sel...Continue Reading

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Citations

Mar 1, 1993·The Journal of Experimental Zoology·C B Barrett, R D Powers
Dec 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·G W RandallC A Shivers
Dec 11, 2002·Theriogenology·A R C L BekerM M Bevers
May 20, 2003·Animal Reproduction Science·M A N Dode, C N Graves
Nov 20, 1982·The Journal of Experimental Zoology·C Racowsky, R W McGaughey
Jan 1, 1991·Endocrine Research·A L Kadam, S S Koide
Jan 26, 2021·Reproductive Medicine and Biology·Hurum MaksuraMohammad Moniruzzaman

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