Specificity of interactions of galectin-3 with Chrp, a cysteine- and histidine-rich cytoplasmic protein

Biochimie
Sulemana BawumiaR C Hughes

Abstract

Earlier work described the cloning of a gene from murine 3T3 cells encoding a cytoplasmic protein Chrp containing a cysteine- and histidine-rich motif characteristic of Zn-finger proteins. The interaction of Chrp with murine galectin-3 first became evident in a yeast two-hybrid screen, but it was also observed in co-precipitation experiments from 3T3 cell lysates. Here, the formation of equimolar complexes by murine Chrp and hamster galectin-3 is shown. Moreover, we found that Chrp binds to the carbohydrate-recognition domain (CRD) of hamster galectin-3 and not to the N-terminal domain carrying the proline- and glycine-rich repeats characteristic of galectin-3 and absent in other galectins. However, galectin-1 does not bind to Chrp, although its CRD is homologous to the galectin-3 CRD. Finally, we report that galectin-3, in a complex with Chrp, binds to laminin in surface plasmon resonance experiments with similar kinetics and affinity as it does in the free state. The formation of higher-order complexes containing these proteins and additional binding partners may be relevant to cytoplasmic functions involving galectin-3.

References

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Citations

Nov 19, 2004·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
May 7, 2008·Biochemistry. Biokhimii︠a︡·E M RapoportN V Bovin
Jul 21, 2009·Biochimica Et Biophysica Acta·Kevin C HaudekEric J Arnoys
Feb 16, 2006·Biochimica Et Biophysica Acta·Jerka DumicMirna Flögel
Sep 9, 2011·Biology of Reproduction·Jennifer D Ly-HuynhDavid A Jans
Jan 24, 2014·International Journal of Oncology·Maria C VladoiuYves St-Pierre
Mar 27, 2018·PeerJ·Dhanasekaran SakthivelTravis Beddoe

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