Specificity of lens initiator tRNA for N-terminal recognition

Molecular Biology Reports
G Strous, H Bloemendal

Abstract

The optimal magnesium ion concentration for chain initiation in a cell-free system derived from bovine eye lens which synthesizes 4 classes of crystallins appears to be 5 mM. In the synthesis of α-crystallin polypeptides which contain one internal methionine residue and the second one in N-terminal position, Met-RNA(fMet) functions exclusively as initiator. On the other hand at 5 mM Mg(2+) Met-tRNA(Met) inserts its methionine into the internal position. However, at higher magnesium ion concentrations the initiator tRNA also donates methionine for chain elongation while at the same time the cell-free system loses its capacity to initiate new polypeptides.

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Citations

Jun 14, 1974·Biochimica Et Biophysica Acta·G J StrousH Bloemendal
Dec 4, 1975·Biochimica Et Biophysica Acta·A J VermorkenH Bloemendal
Dec 1, 1974·Molecular Biology Reports·G J StrousH Bloemedal
Jul 15, 2017·Journal of Biomolecular Structure & Dynamics·Namrata SinghNand Kishore

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