PMID: 9546157Apr 18, 1998Paper

Specificity of milk peptide utilization by Lactococcus lactis

Applied and Environmental Microbiology
V JuillardJ C Gripon

Abstract

To study the substrate specificity of the oligopeptide transport system of Lactococcus lactis for its natural substrates, the growth of L. lactis MG1363 was studied in a chemically defined medium containing milk peptides or a tryptic digest of alpha s2-casein as the source of amino acids. Peptides were separated into acidic, neutral, and basic pools by solid-phase extraction or by cation-exchange liquid chromatogrpaphy. Their ability to sustain growth and the time course of their utilization demonstrated the preferential use of hydrophobic basic peptides with molecular masses ranging between 600 and 1,100 Da by L. lactis MG1363 and the inability to use large, acidic peptides. These peptide utilization preferences reflect the substrate specificity of the oligopeptide transport system of the strain, since no significant cell lysis was inferred. Considering the free amino acid content of milk and these findings on peptide utilization, it was demonstrated that the cessation of growth of L. lactis MG1363 in milk was due to deprivation of leucine and methionine.

References

Aug 1, 1995·Applied and Environmental Microbiology·V JuillardJ Richard
Jan 1, 1994·Advances in Microbial Physiology·J W Payne, M W Smith
Sep 1, 1993·FEMS Microbiology Reviews·B Poolman
Jul 1, 1996·Molecular Microbiology·E R KunjiJ Kok
Oct 1, 1996·Antonie van Leeuwenhoek·E R KunjiW N Konings
Jun 1, 1975·Applied Microbiology·B E Terzaghi, W E Sandine

❮ Previous
Next ❯

Citations

Apr 22, 2006·Applied Microbiology and Biotechnology·Kirsi SavijokiPekka Varmanen
Jan 19, 2010·Pharmaceuticals·Pauliina Jäkälä, Heikki Vapaatalo
Dec 1, 2000·Applied and Environmental Microbiology·B Flambard, V Juillard
Oct 17, 2001·The Journal of Biological Chemistry·Peggy GaraultVeronique Monnet
Feb 12, 2013·Journal of Bacteriology·Rozenn GardanVéronique Monnet
Jun 15, 2011·Journal of Bacteriology·Ronnie P-A BerntssonDirk-Jan Slotboom
Apr 19, 2003·Archives of Microbiology·Peter CurleyD van Sinderen
Jan 18, 2012·Food & Function·Daniel Martínez-MaquedaBlanca Hernández-Ledesma
Sep 9, 2009·Journal of Medicinal Food·Cecilia HaroGraciela Agüero
Jan 30, 1999·Applied and Environmental Microbiology·S FaddaF Toldrá
Dec 28, 2010·Advances in Colloid and Interface Science·Blanca Hernández-LedesmaIsidra Recio
Nov 26, 2020·Food Research International·Yongjun XiaLianzhong Ai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.