PMID: 2498874May 1, 1989Paper

Specificity of mismatch repair following transformation of Saccharomyces cerevisiae with heteroduplex plasmid DNA

Proceedings of the National Academy of Sciences of the United States of America
D K BishopRichard D Kolodner

Abstract

A method is described for genetic detection of mismatch repair products following transformation of Saccharomyces cerevisiae. The method is based on the detection of beta-galactosidase activity in clonal derivatives of cells transformed with heteroduplex plasmid DNA. Heteroduplex plasmid substrates were constructed by insertion of an oligonucleotide heteroduplex into the coding sequence of the Escherichia coli lacZ gene. The plasmid and oligonucleotides were designed so that one strand of the construct would code for a functional beta-galactosidase and the other strand would contain an in-frame nonsense codon. The frequencies of transformed clones containing only Lac+ cells, only Lac- cells, or a mixture of the two Lac phenotypes provided information on the efficiency of the repair reaction. With this method, plasmids carrying single-base substitution mismatches, a single-base frameshift mismatch (T/delta), or a 3-base-pair substitution mismatch (TGA/GAA) were tested. A/C, G/T, G/A, G/G, and T/delta mismatches were repaired with significantly greater efficiencies than C/C, A/A, T/T, and TGA/GAA. T/C was repaired with an intermediate efficiency. The frequencies of products obtained with G/G, G/A, and T/delta mismatches suggested...Continue Reading

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·S FogelF Tavares
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C DohetM Radman
Jan 1, 1987·Nucleic Acids Research·R J Roberts
Jan 1, 1987·Annual Review of Biochemistry·P Modrich
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·C Muster-Nassal, R Kolodner
Aug 11, 1987·Nucleic Acids Research·C P Hodgson, R Z Fisk
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·P J Hastings
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·A L LuP Modrich
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·J L RossignolT Langin
Jun 25, 1982·Journal of Molecular Biology·D T StinchcombR W Davis

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Citations

Jan 1, 1994·Current Genetics·M S EspositoC V Bruschi
Sep 1, 1993·Mutation Research·S E Kohalmi, B A Kunz
Dec 1, 1994·Current Opinion in Biotechnology·R D Kolodner, E Alani
Aug 1, 2000·Mutation Research·R H BortsM F Abdullah
Jan 1, 1997·Cancer Genetics and Cytogenetics·H T LynchJ Lynch
Jan 6, 1999·Mutation Research·J Jiricny
Feb 9, 2006·Chemical Reviews·Ravi R IyerPaul L Modrich
Feb 5, 1999·The Biochemical Journal·G Marra, P Schär
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gina P RodriguezGray F Crouse
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J HolmesP Modrich
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M LichtenA Nicolas
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I VarletP Brooks
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B O Parker, M G Marinus
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V Lauermann, J D Boeke
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·I VarletM Radman
Nov 14, 1997·The EMBO Journal·V Lauermann, J D Boeke
Apr 13, 2011·Genome Biology·Eugenio ManceraLars M Steinmetz
Jun 1, 1995·Current Opinion in Genetics & Development·R Fishel, R D Kolodner
Jul 23, 2009·Mutation Research·Claudia P SpampinatoLuciana D Lario
Feb 8, 2000·The Journal of Biological Chemistry·S GradiaR Fishel
Aug 4, 1995·The Journal of Biological Chemistry·J I RisingerT A Kunkel
Oct 3, 2003·The Journal of Biological Chemistry·Scott D McCullochGuo-Min Li
Sep 5, 2019·International Journal of Molecular Sciences·Yan LiIshita Mukerji
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·F Pâques, J E Haber

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