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Specificity of polyclonal antibodies raised against a novel 24,25-dihydroxyvitamin D3-bovine serum albumin conjugant linked through the C-11alpha or C-3 position

The Journal of Steroid Biochemistry and Molecular Biology

May 1, 1997

N KobayashiK Shimada

PMID: 9366501

Abstract

Novel hapten-carrier conjugants were prepared by coupling 11 alpha-hemiglutaryloxy-(24R)-24,25-dihydroxyvitamin D3 or (24R)-24,25-dihydroxyvitamin D3 [24,25(OH)2D3] 3-hemiglutarate with bovine serum albumin (BSA), to obtain an antibody with high specificity and affinity for use in 24,25...read more

Mentioned in this Paper

Spectrometry, Mass, Fast Atom Bombardment
Protein Binding
Structure-Activity Relationship
Protein NMR Spectroscopy
ALB
Cross Reactions
ABL2
Antigen-Antibody Reactions
ABL2 wt Allele
Immunoassay Method
1
Paper Details
References
  • References15
  • Citations4
12
  • References15
  • Citations4

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Characteristics of antisera to antigenic forms of 1,25-dihydroxycholecalciferol

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Specificity of polyclonal antibodies raised against a novel 24,25-dihydroxyvitamin D3-bovine serum albumin conjugant linked through the C-11alpha or C-3 position

The Journal of Steroid Biochemistry and Molecular Biology

May 1, 1997

N KobayashiK Shimada

PMID: 9366501

DOI:

Abstract

Novel hapten-carrier conjugants were prepared by coupling 11 alpha-hemiglutaryloxy-(24R)-24,25-dihydroxyvitamin D3 or (24R)-24,25-dihydroxyvitamin D3 [24,25(OH)2D3] 3-hemiglutarate with bovine serum albumin (BSA), to obtain an antibody with high specificity and affinity for use in 24,25...read more

Mentioned in this Paper

Spectrometry, Mass, Fast Atom Bombardment
Protein Binding
Structure-Activity Relationship
Protein NMR Spectroscopy
ALB
Cross Reactions
ABL2
Antigen-Antibody Reactions
ABL2 wt Allele
Immunoassay Method
1

Similar Papers Found In These Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry

Characteristics of antisera to antigenic forms of 1,25-dihydroxycholecalciferol

Clinica Chimica Acta; International Journal of Clinical ChemistrySeptember 15, 1986
W B Brown, M Peacock
Journal of Steroid Biochemistry

Recent developments in the measurement of vitamin D and its metabolites in human body fluids

Journal of Steroid BiochemistryDecember 1, 1987
C E PorteousH L Makin
Paper Details
References
  • References15
  • Citations4
12
  • References15
  • Citations4
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