Specificity of ribonucleoprotein interaction determined by RNA folding during complex formulation

Nature
F H AllainGabriele Varani

Abstract

Many proteins involved in pre-mRNA processing contain one or more copies of a 70-90-amino-acid alphabeta module called the ribonucleoprotein domain. RNA maturation depends on the specific recognition by ribonucleoproteins of RNA elements within pre-mRNAs and small nuclear RNAs. The human U1A protein binds an RNA hairpin during splicing, and regulates its own expression by binding an internal loop in the 3'-untranslated region of its pre-mRNA, preventing polyadenylation. Here we report the nuclear magnetic resonance structure of the complex between the regulatory element of the U1A 3'-untranslated region (UTR) and the U1A protein RNA-binding domain. Specific intermolecular recognition requires the interaction of the variable loops of the ribonucleoprotein domain with the well-structured helical regions of the RNA. Formation of the complex then orders the flexible RNA single-stranded loop against the protein beta-sheet surface, and reorganizes the carboxy-terminal region of the protein to maximize surface complementarity and functional group recognition.

References

Aug 25, 1992·Nucleic Acids Research·K B Hall, W T Stump
Jul 3, 1992·Science·J D PuglisiJ R Williamson
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D W HoffmanJ D Keene
Jul 14, 1995·Journal of Molecular Biology·F H Allain, G Varani
Oct 7, 1993·Nature·Y KimP B Sigler
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·I A Laird-Offringa, J G Belasco
Dec 1, 1995·Current Opinion in Structural Biology·A Liljas, M Garber
Mar 1, 1995·Acta Crystallographica. Section D, Biological Crystallography·R Diamond

❮ Previous
Next ❯

Citations

Feb 17, 2001·Biopolymers·David M. J. Lilley
May 20, 1999·Biopolymers·R N De GuzmanM F Summers
May 20, 1999·Biopolymers·D J Patel
Feb 17, 2001·Journal of Molecular Recognition : JMR·A P Demchenko
Aug 25, 2012·Cellular and Molecular Life Sciences : CMLS·Grégoire MasliahFrédéric H-T Allain
Apr 8, 2011·European Biophysics Journal : EBJ·Cyprian D Cukier, Andres Ramos
Nov 18, 2010·Journal of Molecular Modeling·Núria Cerdà-CostaSandra Villegas
Jul 19, 2012·Journal of Biomolecular NMR·Michael F Bardaro, Gabriele Varani
May 10, 2013·Journal of Biomolecular NMR·Robert Kaptein
Mar 21, 1998·Current Opinion in Biotechnology·J Kjems, J Egebjerg
Jun 14, 2000·Current Opinion in Structural Biology·A D Frankel
Feb 17, 2001·Current Opinion in Structural Biology·J M Pérez-Cañadillas, G Varani
Jun 1, 1997·Current Opinion in Structural Biology·E Westhof, D J Patel
Jun 1, 1997·Current Opinion in Structural Biology·A RamosG Varani
Jun 1, 1997·Current Opinion in Structural Biology·K M Weeks
Feb 19, 1999·RNA·C M Reyes, P A Kollman
Aug 12, 1998·Nature Structural Biology·I A Laird-Offringa, J G Belasco
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani
Mar 1, 1997·Nature Structural Biology
Mar 21, 1998·Nature Structural Biology·O C Uhlenbeck
May 20, 1998·Nature Structural Biology·M A Weiss
Jun 26, 2007·Nature Structural & Molecular Biology·Lorenzo CorsiniMichael Sattler
Jan 25, 2005·Nature Structural & Molecular Biology·Satoko YoshizawaKatsumi Maenaka
Oct 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jikui SongMichael D Sheets
Jul 15, 2006·Nucleosides, Nucleotides & Nucleic Acids·Junji KawakamiYoshiatsu Tanabe
Oct 22, 2002·Molecular Biology of the Cell·Petra BjörkLars Wieslander
Oct 6, 1997·The EMBO Journal·F H AllainG Varani
Feb 21, 1998·The EMBO Journal·S E ButcherJ Feigon
Mar 4, 2000·The EMBO Journal·A RamosG Varani
Jul 1, 1997·Nucleic Acids Research·A Slama-SchwokJ M Lehn
Oct 1, 1998·Nucleic Acids Research·L R ComolliI Tinoco
Dec 24, 1998·Nucleic Acids Research·D E Draper, L P Reynaldo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.