Specificity of the cyanobacterial orange carotenoid protein: influences of orange carotenoid protein and phycobilisome structures

Plant Physiology
Denis JalletDiana Kirilovsky

Abstract

Cyanobacteria have developed a photoprotective mechanism that decreases the energy arriving at the reaction centers by increasing thermal energy dissipation at the level of the phycobilisome (PB), the extramembranous light-harvesting antenna. This mechanism is triggered by the photoactive Orange Carotenoid Protein (OCP), which acts both as the photosensor and the energy quencher. The OCP binds the core of the PB. The structure of this core differs in diverse cyanobacterial strains. Here, using two isolated OCPs and four classes of PBs, we demonstrated that differences exist between OCPs related to PB binding, photoactivity, and carotenoid binding. Synechocystis PCC 6803 (hereafter Synechocystis) OCP, but not Arthrospira platensis PCC 7345 (hereafter Arthrospira) OCP, can attach echinenone in addition to hydroxyechinenone. Arthrospira OCP binds more strongly than Synechocystis OCP to all types of PBs. Synechocystis OCP can strongly bind only its own PB in 0.8 m potassium phosphate. However, if the Synechocystis OCP binds to the PB at very high phosphate concentrations (approximately 1.4 m), it is able to quench the fluorescence of any type of PB, even those isolated from strains that lack the OCP-mediated photoprotective mechani...Continue Reading

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Citations

Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kun TangKai-Hong Zhao
Mar 15, 2015·Scientific Reports·Céline Bourcier de CarbonDiana Kirilovsky
Aug 6, 2015·Biophysical Journal·Eugene G MaksimovAndrew B Rubin
Dec 3, 2016·Nature Plants·Diana Kirilovsky, Cheryl A Kerfeld
Jan 12, 2017·Biophysical Journal·Eugene G MaksimovAndrew B Rubin
Jul 5, 2017·The New Phytologist·Cheryl A KerfeldMaria Agustina Dominguez-Martin
Sep 19, 2019·Nature Plants·Fernando MuzzopappaDiana Kirilovsky
Sep 13, 2017·Scientific Reports·Petr VítekJacek Wierzchos
Jan 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Wenjing LouHaijun Liu
Apr 10, 2017·Current Opinion in Plant Biology·Han BaoCheryl A Kerfeld
Jun 23, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Chakib DjediatCécile Bernard

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