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Specificity of the polyclonal antibodies raised against a novel 25-hydroxyvitamin D3-bovine serum albumin conjugate linked through the C-11 alpha position

The Journal of Steroid Biochemistry and Molecular Biology

Apr 1, 1994

N KobayashiK Shimada

PMID: 8180121

Abstract

To obtain a specific antibody for use in 25-hydroxyvitamin D3 [25(OH)D3] immunoassay, a novel hapten-carrier conjugate was prepared by coupling 11 alpha-hemiglutaryloxy-25(OH)D3 with bovine serum albumin (BSA). Three polyclonal antibodies (Ab11) showing high titer and affinity for 25(OH...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
Ergocalciferol
ALB
Cross Reactions
Vitamin D
Immunization Domain
Antigens
Vitamin D [EPC]
Immunoassay Method
D Vitamin
2
Paper Details
References
  • References14
  • Citations3
12

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Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

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Specificity of the polyclonal antibodies raised against a novel 25-hydroxyvitamin D3-bovine serum albumin conjugate linked through the C-11 alpha position

The Journal of Steroid Biochemistry and Molecular Biology

Apr 1, 1994

N KobayashiK Shimada

PMID: 8180121

DOI:

Abstract

To obtain a specific antibody for use in 25-hydroxyvitamin D3 [25(OH)D3] immunoassay, a novel hapten-carrier conjugate was prepared by coupling 11 alpha-hemiglutaryloxy-25(OH)D3 with bovine serum albumin (BSA). Three polyclonal antibodies (Ab11) showing high titer and affinity for 25(OH...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
Ergocalciferol
ALB
Cross Reactions
Vitamin D
Immunization Domain
Antigens
Vitamin D [EPC]
Immunoassay Method
D Vitamin
2

Similar Papers Found In These Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry

Evaluation of solubilizing agents for 25-hydroxy-vitamin D3 immunoassays

Clinica Chimica Acta; International Journal of Clinical ChemistryJuly 31, 1992
N KobayashiK Shimada
Paper Details
References
  • References14
  • Citations3
12
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