Specificity of the synergistic anion for iron binding by ferric binding protein from Neisseria gonorrhoeae

Biochemistry
Elena G BekkerMichael E P Murphy

Abstract

Ferric binding protein in Neisseria gonorrhoeae (nFbpA) transports iron from outer membrane receptors for host proteins across the periplasm to a permease in an alternative pathway to the use of siderophores in some pathogenic bacteria. Phosphate and nitrilotriacetate, both at pH 8, and vanadate at pH 9 are shown to be synergistic in promoting ferric binding to nFbpA, in contrast to carbonate and sulfate. Interestingly, only phosphate produces the fully closed conformation of nFbpA as defined by native electrophoresis. The role of phosphate was probed by constructing three mutants: Q58E, Q58R, and G140H. The anion and iron binding properties of the Q58E mutant are similar to the wild-type protein, implying that one phosphate oxygen is a hydrogen bond donor and may in part define the specificity of nFbpA for phosphate over sulfate. Phosphate is a weakly synergistic anion in the Q58R and G140H mutants, and these mutants do not form completely closed structures. Ferric binding was investigated by both isothermal titration and differential scanning calorimetry. The apparent affinity of nFbpA for iron in a solution of 30 mM citrate is 1 order of magnitude larger in the presence (K(app)= 1.7 x 10(5) M(-1)) of phosphate than in its ab...Continue Reading

References

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Oct 11, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hakim BoukhalfaAlvin L Crumbliss

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Citations

Feb 22, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Arindam MukherjeePeter J Sadler
Oct 13, 2005·Journal of Molecular Recognition : JMR·Abdessamad Ababou, John E Ladbury
Sep 22, 2011·Biochimica Et Biophysica Acta·Claire J Parker SiburtAlvin L Crumbliss
Jan 17, 2015·Inorganic Chemistry·Jacqueline F CawthrayChris Orvig
Oct 3, 2017·Metallomics : Integrated Biometal Science·H AbdizadehB Dedeoglu
Dec 23, 2004·The Journal of Biological Chemistry·Stacey A L Tom-YewMichael E P Murphy
Sep 27, 2006·Inorganic Chemistry·Jonathan P HillKatsuhiko Ariga
Apr 24, 2020·Journal of Chemical Theory and Computation·Farzaneh JalalypourCanan Atilgan

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