PMID: 6165330Apr 1, 1981Paper

Specificity of translation of spore polypeptides in bacillus in vitro systems

Archives of Microbiology
W Wright, A I Aronson

Abstract

Cell free extracts prepared from exponentially growing Escherichia coli and Bacillus cereus as well as from Bacillus cereus at the end of exponential growth were optimized for various factors required for amino acid incorporation when programmed with Bacillus ribonucleic acid. All three preparations synthesized glutamine synthetase antigen when ribonucleic acid from a Bacillus subtilis strain that overproduces glutamine synthetase was added. The post exponential Bacillus cereus extract, however, was most active for the synthesis of Bacillus cereus sport coat antigen when supplemented with the appropriate ribonucleic acid. There appears to be some specificity in the translation of at least this sporulation messenger RNA.

References

Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y S Cheng, A I Aronson
Jun 1, 1979·Archives of Microbiology·H A DouthitR A Preston
Oct 1, 1977·Journal of Bacteriology·G H Chambliss, L Legault-Demare
Dec 31, 1976·Molecular & General Genetics : MGG·L Legualt-Démare, G H Chambliss
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
May 1, 1961·Virology·H FRAENKEL-CONRATA TSUGITA
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

Related Concepts

Bacillus cereus
Natto Bacteria
Bacterial Proteins
Cell-Free System
Alkalescens-Dispar Group
Glutamate-Ammonia Ligase
RNA, Bacterial
Spores, Bacterial
Protein Biosynthesis

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