Specificity of TRAP-RNA interactions: crystal structures of two complexes with different RNA sequences

Acta Crystallographica. Section D, Biological Crystallography
Nicholas H HopcroftAlfred A Antson

Abstract

The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes in bacilli by binding to the leader region of the nascent trp operon mRNA. When activated by binding tryptophan, the 11-subunit circular TRAP molecule binds to a target sequence consisting of 11 (G/U)AG repeats, separated by two or three variable 'spacer' nucleotides. Reported here are two crystal structures of TRAP bound to RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the spacer regions of the RNA molecules to be highly flexible, making no direct hydrogen-bonding contacts with the protein. Comparison of these structures with the previous structure of TRAP bound to (GAGAU)(10)GAG RNA, in which the spacer nucleotides stack with each other close to the protein surface, shows that the RNA can adopt different conformations depending on the sequence of the spacer regions. This gives insight into the structural basis of the specificity of TRAP and into the mechanism of binding.

Citations

Apr 6, 2005·Nature Structural & Molecular Biology·Paul Gollnick, Alfred Antson
Nov 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mikhail B ShevtsovAlfred A Antson
Mar 13, 2008·Journal of Molecular Biology·Yanling Chen, Paul Gollnick
Oct 3, 2007·Proteins·Jonathan J Ellis, Susan Jones
Feb 22, 2008·Protein Science : a Publication of the Protein Society·Masahiro WatanabeJonathan G Heddle
Dec 14, 2004·Journal of Molecular Biology·Maria V BarbolinaPaul Gollnick
Apr 22, 2004·Journal of Molecular Biology·Doug SnyderJames L Cole
Mar 31, 2004·Journal of Molecular Biology·Nicholas H HopcroftAlfred A Antson
Dec 24, 2010·The Biochemical Journal·Ali D MalayJeremy R H Tame
Nov 16, 2005·Annual Review of Genetics·Paul GollnickCharles Yanofsky
May 4, 2016·Acta Crystallographica. Section D, Structural Biology·Charles S BuryElspeth F Garman

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