Specificity, sensitivity, and operability of RSID™-urine for forensic identification of urine: comparison with ELISA for Tamm-Horsfall protein

Journal of Forensic Sciences
Tomoko AkutsuKoichi Sakurada

Abstract

In this study, the specificity, sensitivity, and operability of RSID™-Urine, a new immunochromatographic test for urine identification, was evaluated and compared with ELISA detection of Tamm-Horsfall protein (THP). Urine was successfully identified among other body fluids using RSID™-Urine and ELISA detection of THP. The detection limit of RSID™-Urine equated to 0.5 μL of urine; although the sensitivity of RSID™-Urine may be lower than that of ELISA detection of THP, it is thought to be sufficient for application to casework samples. However, results from RSID™-Urine must be interpreted with caution when the sample may have been contaminated with blood or vaginal fluid, because this might inhibit urine detection. The RSID™-Urine assay can be performed in just 15 min by dropping the extracted sample onto the test cassette. Therefore, RSID™-Urine should be an effective tool for the forensic identification of urine, in addition to ELISA detection of THP.

References

Jan 1, 1983·Journal - Forensic Science Society·M C Taylor, J S Hunt
May 1, 1950·Proceedings of the Society for Experimental Biology and Medicine·I TAMM, F L HORSFALL
Dec 3, 2009·International Journal of Legal Medicine·Tomoko AkutsuKoichi Sakurada
Mar 6, 2010·Journal of Forensic Sciences·Tomoko AkutsuKoichi Sakurada

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Citations

May 20, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·The Son LeShinya Maenosono

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