SPECT/CT Imaging of Mycobacterium tuberculosis Infection with [125 I]anti-C3d mAb

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Catherine A FossMartin G Pomper

Abstract

Diagnosis and therapeutic monitoring of chronic bacterial infection requires methods to detect and localize sites of infection accurately. Complement C3 activation fragments are generated and covalently bound to selective bacterial pathogens during the immune response and can serve as biomarkers of ongoing bacterial infection. We have developed several probes for detecting tissue-bound C3 deposits, including a monoclonal antibody (mAb 3d29) that recognizes the tissue-bound terminal processing fragments iC3b and C3d but does not recognize native circulating C3 or tissue-bound C3b. To determine whether mAb 3d29 could be used to detect chronic Mycobacterium tuberculosis infection non-invasively, aerosol-infected female C3HeB/FeJ mice were injected with [125I]3d29 mAb and either imaged using single-photon emission computed tomography (SPECT)/X-ray computed tomography (CT) imaging at 24 and 48 h after radiotracer injection or being subjected to biodistribution analysis. Discrete lesions were detected by SPECT/CT imaging in the lungs and spleens of infected mice, consistent with the location of granulomas in the infected animals as detected by CT. Low-level signal was seen in the spleens of uninfected mice and no signal was seen in t...Continue Reading

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Citations

Dec 4, 2019·Frontiers in Immunology·Maedeh MohebnasabBo Nilsson
Feb 6, 2020·International Journal of Molecular Sciences·Chiara GiraudoMassimiliano Casali
May 21, 2020·Physics in Medicine and Biology·Paul LecoqMathieu Benoit
Mar 2, 2021·The Journal of Clinical Investigation·Alvaro A OrdonezSanjay K Jain
Apr 14, 2020·Tuberculosis·Michelle H LarsenRichard T Robinson
Dec 18, 2021·Human Molecular Genetics·Chao-Jen WongReSolve Network

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