PMID: 2118161Aug 28, 1990Paper

Spectophotometric quantification of cell-cell adherence by an enzyme-linked immuno-cell adhesion assay

Journal of Immunological Methods
I BruynzeelR Willemze

Abstract

In the present study a spectophotometric method was developed to determine the number of T cells binding to human epidermal keratinocytes (KC). In this cell adhesion assay the adherent T cells were detected with a cell-specific monoclonal antibody conjugated to horseradish peroxidase and the coloured substrate quantified in an ELISA reader at 492 nm. A correlation was demonstrated between the number of T cells and the extinction values measured. The enzyme-linked immuno-cell adhesion assay (ELICAA) was used to quantify KC/T lymphocyte adherence in a series of experiments designed to evaluate its reliability and reproducibility. Compared with the 51Cr-labelled adherence assay, the ELICAA was a safe, rapid and accurate method avoiding the use of radioactive material.

References

Jan 1, 1979·Journal of Immunological Methods·D M Boorsma, J G Streefkerk
May 25, 1988·Journal of Immunological Methods·D M LewinsohnE C Butcher
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R GambleM A Vadas
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D WrightS C Silverstein

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Citations

Jul 5, 1991·Journal of Immunological Methods·R Pearce-PrattA S Bourinbaiar
Sep 15, 1993·Journal of Immunological Methods·I MikiH Kase
Apr 16, 1998·The British Journal of Dermatology·M C LittleS Mac Neil

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