Spectral analysis of doxorubicin accumulation and the indirect quantification of its DNA intercalation

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Ondřej HovorkaBlanka Ríhová

Abstract

There is a wide range of techniques utilizing fluorescence of doxorubicin (Dox) commonly used for analysis of intracellular accumulation and destiny of various drug delivery systems containing this anthracycline antibiotic. Unfortunately, results of these studies can be significantly influenced by doxorubicin degradation product, 7,8-dehydro-9,10-desacetyldoxorubicinone (D*) forming spontaneously in aqueous environment, whose fluorescence strongly interfere with that of doxorubicin. Here, we define two microscopy techniques enabling to distinguish and separate Dox and D* emission based either on its spectral properties or on fluorescence lifetime analysis. To analyze influx and nuclear accumulation of Dox (free or polymer-bound) by flow cytometry, we propose using an indirect method based on its DNA intercalation competition with Hoechst 33342 rather than a direct measurement of doxorubicin fluorescence inside the cells.

References

Aug 13, 1998·Biophysical Chemistry·K K KarukstisR J Rosenfeld
Jan 21, 2000·Journal of Controlled Release : Official Journal of the Controlled Release Society·K UlbrichB Ríhová
Apr 8, 2003·Current Medicinal Chemistry. Anti-cancer Agents·M BinaschiF Animati
Jan 20, 2007·Biochemical Society Transactions·R Duncan
Nov 6, 2007·Current Drug Delivery·Abdus SamadM Aqil
Mar 8, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·B RíhováK Ulbrich
Sep 9, 2008·International Journal of Pharmaceutics·Raymond M Schiffelers, Gert Storm
Oct 16, 2008·Current Topics in Medicinal Chemistry·Istvan J MajorosJames R Baker
May 9, 2009·International Journal of Oncology·Leonard W SeymourJames Cassidy
May 23, 2009·Cancer Science·Yasuhiro Matsumura, Kazunori Kataoka

❮ Previous
Next ❯

Citations

Aug 15, 2012·Journal of Physiology and Biochemistry·Reid HaywardTraci Parry
Sep 8, 2015·Journal of Pharmaceutical Sciences·Katie F MaassKarl Dane Wittrup
Sep 19, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Suvi K SoininenMarika Ruponen
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ariane Rocha BartolomeuJosé Maurício Sforcin
Jul 15, 2015·Biomacromolecules·Richard LagaMichal Pechar
Oct 15, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Michela BelliniDavide Prosperi
Jun 29, 2017·Expert Review of Anticancer Therapy·Eric Chekwube AniogoHeidi Abrahamse
Jun 24, 2016·Physical Chemistry Chemical Physics : PCCP·Saswat MohapatraKankan Bhattacharyya
Dec 1, 2017·Journal of Biomedical Optics·Marjorie CarlsonPaolo P Provenzano
Dec 21, 2018·PLoS Computational Biology·Vlad ElgartJoseph Loscalzo
Jan 27, 2012·Macromolecular Bioscience·Kirk D Fowers, Jindřich Kopeček
Sep 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Viktorija HercegNorbert Lange
Mar 16, 2018·Oxidative Medicine and Cellular Longevity·Bassant M BarakatAlaaeldeen M Elbahaie
Jan 27, 2019·Cellular Oncology (Dordrecht)·Hara PolioudakiPanayiotis A Theodoropoulos
Jan 24, 2013·Physical Chemistry Chemical Physics : PCCP·Pascale Changenet-BarretSandra Monti
Feb 25, 2021·Scientific Reports·Paul Christopher LyonRobert Carlisle
Apr 13, 2012·The Journal of Physical Chemistry. a·Krzysztof NawaraG J Blanchard
Oct 26, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Danielle van der WesthuizenOrde Q Munro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.