Spectral backscattering properties of marine phytoplankton cultures

Optics Express
Amanda L WhitmireTimothy J Cowles

Abstract

The backscattering properties of marine phytoplankton, which are assumed to vary widely with differences in size, shape, morphology and internal structure, have been directly measured in the laboratory on a very limited basis. This work presents results from laboratory analysis of the backscattering properties of thirteen phytoplankton species from five major taxa. Optical measurements include portions of the volume scattering function (VSF) and the absorption and attenuation coefficients at nine wavelengths. The VSF was used to obtain the backscattering coefficient for each species, and we focus on intra- and interspecific variability in spectral backscattering in this work. Ancillary measurements included chlorophyll-a concentration, cell concentration, and cell size, shape and morphology via microscopy for each culture. We found that the spectral backscattering properties of phytoplankton deviate from theory at wavelengths where pigment absorption is significant. We were unable to detect an effect of cell size on the spectral shape of backscattering, but we did find a relationship between cell size and both the backscattering ratio and backscattering cross-section. While particulate backscattering at 555 nm was well correlat...Continue Reading

References

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May 28, 2007·Optics Express·A L WhitmireW S Pegau
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Citations

Jul 16, 2011·Analytical Chemistry·Jeffrey S EricksonFrances S Ligler
Oct 6, 2012·Optics Express·Robert J W BrewinNick J Hardman-Mountford
Jan 22, 2015·Optics Express·Xiaodong ZhangDeric J Gray
Dec 24, 2018·Nature Communications·Emanuele OrganelliAnnick Bricaud
Sep 15, 2015·Applied Optics·Wayne Homer Slade, Emmanuel Boss
Jun 15, 2012·Applied Optics·Tihomir Sabinov KostadinovNathalie Guillocheau
Jan 3, 2018·Frontiers in Marine Science·Watson W Gregg, Cécile S Rousseaux
May 14, 2020·Applied Optics·James H Churnside, Joseph A Shaw
Mar 11, 2021·Applied Optics·Xiaodong ZhangYuanheng Xiong

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