PMID: 11332877May 3, 2001Paper

Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating. Analysis of high-temperature fluorescence rise around 60 degrees C

Journal of Photochemistry and Photobiology. B, Biology
Petr IlíkF Vácha

Abstract

The spectral characteristics of chlorophyll fluorescence and absorption during linear heating of barley leaves within the range 25-75 degreesC (fluorescence temperature curve, FTC) were studied. Leaves with various content of light harvesting complexes (green, Chl b-less chlorina f2 and intermittent light grown) revealing different types of FTC were used. Differential absorption, emission and excitation spectra documented four characteristic phases of the FTC. The initial two FTC phases (a rise in the 46-49 degreesC region and a subsequent decrease to about 55 degreesC) mostly reflected changes in the fluorescence quantum yield peaking at about 685 nm. A steep second fluorescence rise at 55-61 degreesC was found to originate from a short-wavelength Chl a spectral form (emission maximum at 675 nm) causing a gradual blue shift of the emission spectra. In this temperature range, a clear correspondence of the blue shift in the emission and absorption spectra was found. We suggest that the second fluorescence rise in FTC reflects a weakening of the Chl a-protein interaction in the thylakoid membrane.

Citations

Jul 15, 2003·Photochemistry and Photobiology·Petr IlíkJan Naus
Sep 19, 2012·Journal of Biophotonics·Hans Georg BreunigKarsten König
May 21, 2011·The Plant Journal : for Cell and Molecular Biology·Simona BirticMichel Havaux
Aug 29, 2009·Biochimica Et Biophysica Acta·Lenka LípováPetr Ilík

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