Spectral characterization of the recombinant mouse tumor suppressor 101F6 protein.

European Biophysics Journal : EBJ
A BèrcziH Asard

Abstract

Tumor suppressor protein 101F6, a gene product of the 3p21.3 (human) and 9F1 (mouse) chromosomal region, has recently been identified as a member of the cytochrome b561 (Cyt-b561) protein family by sequence homology. The His(6)-tagged recombinant mouse tumor suppressor Cyt-b561 protein (TSCytb) was recently expressed in yeast and purified, and the ascorbate reducibility was determined. TSCytb is auto-oxidizable and has two distinct heme b centers with redox potentials of approximately 40 and approximately 140 mV. Its split alpha-band in the dithionite-reduced spectrum at both 295 and 77 K is well resolved, and the separation between the two alpha-peaks is approximately 7 nm (approximately 222 cm(-1)). Singular value decomposition analysis of the split alpha-band in the ascorbate-reduced spectra revealed the presence of two major spectral components, each of them with split alpha-band but with different peak separations (6 and 8 nm). Similar minor differences in peak separation were obtained when the split alpha-bands in ascorbate-reduced difference spectra at low (<1 mM) and high (>10 mM) ascorbate concentrations were analysed. According to low-temperature electron paramagnetic resonance (EPR) spectroscopy, the two heme b cente...Continue Reading

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Citations

Dec 20, 2012·Antioxidants & Redox Signaling·Han AsardAlajos Bérczi
Apr 25, 2012·European Biophysics Journal : EBJ·Alajos BércziHan Asard
Jan 18, 2011·FEBS Letters·Filip DesmetSabine Van Doorslaer
Feb 10, 2016·The Journal of Biological Chemistry·Sebastian PintscherArtur Osyczka

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