Spectral confocal imaging of fluorescently tagged nicotinic receptors in knock-in mice with chronic nicotine administration.

Journal of Visualized Experiments : JoVE
Anthony Renda, Raad Nashmi

Abstract

Ligand-gated ion channels in the central nervous system (CNS) are implicated in numerous conditions with serious medical and social consequences. For instance, addiction to nicotine via tobacco smoking is a leading cause of premature death worldwide (World Health Organization) and is likely caused by an alteration of ion channel distribution in the brain. Chronic nicotine exposure in both rodents and humans results in increased numbers of nicotinic acetylcholine receptors (nAChRs) in brain tissue. Similarly, alterations in the glutamatergic GluN1 or GluA1 channels have been implicated in triggering sensitization to other addictive drugs such as cocaine, amphetamines and opiates. Consequently, the ability to map and quantify distribution and expression patterns of specific ion channels is critically important to understanding the mechanisms of addiction. The study of brain region-specific effects of individual drugs was advanced by the advent of techniques such as radioactive ligands. However, the low spatial resolution of radioactive ligand binding prevents the ability to quantify ligand-gated ion channels in specific subtypes of neurons. Genetically encoded fluorescent reporters, such as green fluorescent protein (GFP) and its...Continue Reading

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Citations

May 16, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Kumar Selvarajoo
Feb 11, 2015·Neuropharmacology·Brandon J Henderson, Henry A Lester
Dec 3, 2014·Neuropharmacology·Jingyi WangJon Lindstrom
Jul 13, 2019·Analytical Chemistry·Xu FuChristopher I Richards

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