Spectral editing at ultra-fast magic-angle-spinning in solid-state NMR: facilitating protein sequential signal assignment by HIGHLIGHT approach

Journal of Biomolecular NMR
Songlin WangYoshitaka Ishii

Abstract

This study demonstrates a novel spectral editing technique for protein solid-state NMR (SSNMR) to simplify the spectrum drastically and to reduce the ambiguity for protein main-chain signal assignments in fast magic-angle-spinning (MAS) conditions at a wide frequency range of 40-80 kHz. The approach termed HIGHLIGHT (Wang et al., in Chem Comm 51:15055-15058, 2015) combines the reverse (13)C, (15)N-isotope labeling strategy and selective signal quenching using the frequency-selective REDOR pulse sequence under fast MAS. The scheme allows one to selectively observe the signals of "highlighted" labeled amino-acid residues that precede or follow unlabeled residues through selectively quenching (13)CO or (15)N signals for a pair of consecutively labeled residues by recoupling (13)CO-(15)N dipolar couplings. Our numerical simulation results showed that the scheme yielded only ~15% loss of signals for the highlighted residues while quenching as much as ~90% of signals for non-highlighted residues. For lysine-reverse-labeled micro-crystalline GB1 protein, the 2D (15)N/(13)Cα correlation and 2D (13)Cα/(13)CO correlation SSNMR spectra by the HIGHLIGHT approach yielded signals only for six residues following and preceding the unlabeled ly...Continue Reading

References

Jan 1, 1988·Annual Review of Biochemistry·S B Kent
Mar 1, 1997·Journal of Magnetic Resonance·M Bak, N C Nielsen
Jan 5, 2000·Journal of Magnetic Resonance·B M FungK Ermolaev
Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aneta T PetkovaRobert Tycko
Jun 18, 2003·Journal of Magnetic Resonance·Corey R Morcombe, Kurt W Zilm
May 27, 2004·Journal of the American Chemical Society·Tatyana I IgumenovaA Joshua Wand
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Henrike HeiseMarc Baldus
Dec 13, 2005·Journal of Magnetic Resonance·Mikhail Veshtort, Robert G Griffin
Jul 11, 2006·Magnetic Resonance in Chemistry : MRC·David StauntonIain D Campbell
Dec 7, 2007·Nature Structural & Molecular Biology·Sandra ChimonYoshitaka Ishii
Dec 21, 2007·Journal of the American Chemical Society·Matthias HillerHartmut Oschkinat
Apr 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jonathan J HelmusChristopher P Jaroniec
Feb 10, 2009·Nature Methods·Nalinda P WickramasingheYoshitaka Ishii
Feb 27, 2009·Journal of Molecular Biology·Lichi ShiVladimir Ladizhansky
Feb 28, 2009·Annual Review of Biophysics·Ann McDermott
Apr 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anant K ParavastuRobert Tycko
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei QiangDavid P Weliky
Jun 24, 2010·Chembiochem : a European Journal of Chemical Biology·Anne SchuetzBeat H Meier
Jul 6, 2010·Journal of Molecular Biology·Manasi P BhateAnn E McDermott
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Luise RichterGerd Multhaup
Dec 15, 2010·Journal of the American Chemical Society·Jinfeng LaiLeonard J Mueller
Mar 9, 2011·Journal of the American Chemical Society·V N SivanandamPatrick C A van der Wel
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Raffaello VerardiGianluigi Veglia
Jul 20, 2011·Journal of the American Chemical Society·Józef R LewandowskiRobert G Griffin
May 9, 2012·Angewandte Chemie·Juan Miguel Lopez del AmoBernd Reif
Jun 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Michael J KnightGuido Pintacuda
Nov 7, 2012·Nature·Soo Bin Park
Nov 15, 2012·Scientific Reports·Shakeel A ShahidBarth-Jan van Rossum
May 28, 2013·The Journal of Biological Chemistry·Shivani AhujaAyyalusamy Ramamoorthy
May 6, 2015·Nature Structural & Molecular Biology·Yiling XiaoYoshitaka Ishii

❮ Previous
Next ❯

Citations

Jan 10, 2021·Scientific Reports·Kathryn P ScherpelzStephen C Meredith
Jan 29, 2021·Solid State Nuclear Magnetic Resonance·Yi JiGuangjin Hou
Dec 11, 2017·Journal of Magnetic Resonance·Yoshitaka IshiiTakayuki Kamihara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.