Spectral filtering modulation method for estimation of hemoglobin concentration and oxygenation based on a single fluorescence emission spectrum in tissue phantoms.

Medical Physics
Quan Liu, Tuan Vo-Dinh

Abstract

Hemoglobin concentration and oxygenation in tissue are important biomarkers that are useful in both research and clinical diagnostics of a wide variety of diseases such as cancer. The authors aim to develop simple ratiometric method based on the spectral filtering modulation (SFM) of fluorescence spectra to estimate the total hemoglobin concentration and oxygenation in tissue using only a single fluorescence emission spectrum, which will eliminate the need of diffuse reflectance measurements and prolonged data processing as required by most current methods, thus enabling rapid clinical measurements. The proposed method consists of two steps. In the first step, the total hemoglobin concentration is determined by comparing a ratio of fluorescence intensities at two emission wavelengths to a calibration curve. The second step is to estimate oxygen saturation by comparing a double ratio that involves three emission wavelengths to another calibration curve that is a function of oxygen saturation for known total hemoglobin concentration. Theoretical derivation shows that the ratio in the first step is linearly proportional to the total hemoglobin concentrations and the double ratio in the second step is related to both total hemoglob...Continue Reading

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Citations

Jan 18, 2012·Cellular and Molecular Life Sciences : CMLS·Ruslan I Dmitriev, Dmitri B Papkovsky
May 24, 2011·World Journal of Clinical Oncology·Quan Liu
May 30, 2014·Journal of Biomedical Optics·Shaiju S NazeerRamapurath S Jayasree
Apr 25, 2015·Journal of Biophotonics·Wenfeng WangQuan Liu
Dec 15, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S RameshRamapurath S Jayasree

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