Spectral imaging to measure heterogeneity in membrane lipid packing.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Erdinc SezginChristian Eggeling

Abstract

Physicochemical properties of the plasma membrane have been shown to play an important role in cellular functionality. Among those properties, the molecular order of the lipids, or the lipid packing, is of high importance. Changes in lipid packing are believed to compartmentalize cellular signaling by initiating coalescence and conformational changes of proteins. A common way to infer membrane lipid packing is by using membrane-embedded polarity-sensitive dyes, whose emission spectrum is dependent on the molecular order of the immediate membrane environment. Here, we report on an improved determination of such spectral shifts in the emission spectrum of the polarity-sensitive dyes. This improvement is based on the use of spectral imaging on a scanning confocal fluorescence microscope in combination with an improved analysis, which considers the whole emission spectrum instead of just single wavelength ranges. Using this approach and the polarity-sensitive dyes C-Laurdan or Di-4-ANEPPDHQ, we were able to image-with high accuracy-minute differences in the lipid packing of model and cellular membranes.

References

Feb 27, 2001·Biophysical Journal·C DietrichE Gratton
Oct 6, 2005·The Journal of Cell Biology·Katharina GausThomas Harder
Feb 16, 2007·Chembiochem : a European Journal of Chemical Biology·Hwan Myung KimBong Rae Cho
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tobias BaumgartWatt W Webb
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hermann-Josef KaiserKai Simons
Jan 2, 2010·Science·Daniel Lingwood, Kai Simons
Sep 24, 2010·Nature Reviews. Molecular Cell Biology·Kai Simons, Mathias J Gerl
Nov 15, 2011·Biochemical and Biophysical Research Communications·Jesper Søndergaard Hansen, Claus Hélix-Nielsen
Dec 14, 2011·Nature Protocols·Dylan M OwenKatharina Gaus
Dec 21, 2011·Journal of Lipid Research·Martín M Dodes TraianValeria Levi
Mar 28, 2012·Biochimica Et Biophysica Acta·Erdinc SezginPetra Schwille
Aug 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·James Peter SáenzKai Simons
Jan 15, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Justyna Barucha-KraszewskaChristophe Ramseyer
Jul 23, 2013·Biochimica Et Biophysica Acta·Jorge Bernardino de la SernaLuis A Bagatolli
Mar 19, 2014·Cell·Malte Schmick, Philippe I H Bastiaens
Jun 7, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Erdinc SezginKai Simons
Oct 22, 2014·Methods in Molecular Biology·Ottavia GolfettoEnrico Gratton

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Citations

Dec 15, 2015·Biochimica Et Biophysica Acta·Remigiusz WorchMarzena Jankowska-Anyszka
Apr 28, 2016·Biophysical Journal·Kandice R LeventalIlya Levental
Jun 9, 2016·Biophysical Journal·Olena SteshenkoChristian Eggeling
Feb 15, 2017·Nature Communications·M FritzscheC Eggeling
Jun 25, 2017·Cell Biochemistry and Biophysics·Yuri L NemotoTakahiro K Fujiwara
Feb 17, 2018·Journal of Physics D: Applied Physics·Mariana AmaroErdinc Sezgin
Sep 14, 2018·Journal of Cell Science·Edward JenkinsErdinc Sezgin
Nov 22, 2018·Soft Matter·Shamit ShrivastavaMatthias F Schneider
Sep 8, 2019·Scientific Reports·Manjari MishraShobhna Kapoor
Mar 31, 2017·Nature Reviews. Molecular Cell Biology·Erdinc SezginChristian Eggeling
Mar 17, 2018·Cell Death & Disease·Danuta Gutowska-OwsiakGraham S Ogg
Sep 17, 2017·Nature Communications·Jakub ChojnackiChristian Eggeling
Apr 16, 2020·International Journal of Molecular Sciences·Bingen G MonasterioAlicia Alonso
Apr 3, 2019·Frontiers in Microbiology·Philippe CalvezAndré Zapun
Jan 9, 2018·Polymers·Radu TanasescuAndreas Zumbuehl
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Katharina ReglinskiRalf Erdmann
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Danuta Gutowska-OwsiakJorge Bernardino de la Serna
Oct 22, 2019·Biophysical Journal·Michael L SalinasRobert S Chapkin
May 4, 2020·Biophysical Journal·Aurélien BarbotinErdinc Sezgin
Apr 5, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Florina ZakanyZoltan Varga
Sep 14, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Miles AronEleanor Stride
Oct 27, 2018·Chemical Reviews·Marek CebecauerMartin Hof
Oct 13, 2020·Analytical Chemistry·Dmytro I DanylchukAndrey S Klymchenko
Feb 7, 2020·The Journal of Physical Chemistry. B·Daniel BeckersErdinc Sezgin
Dec 20, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Richard J BrowningEleanor Stride
Apr 11, 2017·ACS Omega·Valerio PerenoEleanor Stride
Nov 18, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Marcos Arribas Perez, Paul A Beales

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Methods Mentioned

BETA
Fluorescence
FCS

Software Mentioned

ImageJ
Fiji
ImageJ CurveFitter

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