Jan 1, 1985

Spectral interactions of piperonyl butoxide and isocyanides with purified hepatic cytochrome P-450 from uninduced mice

General Pharmacology
G A BeumelE Hodgson

Abstract

The binding of isocyanides and the metabolites of piperonyl butoxide (PBO) to reduced cytochrome P-450 in intact microsomes gives rise to the type III optical difference spectrum which is characterized by two pH dependent peaks in the Soret region. Each of the purified cytochrome P-450 isozymes (A1, B1, B2, B3) metabolized PBO and produced a spectrum in the Soret region. Only the A1 fraction produced the pH dependent type III spectrum. The B1 fraction produced a spectrum with only one peak at 430 nm while the spectrum produced by both the B2 and B3 fractions contained only the 455 nm peak. Each of the isozymes produce pH dependent type III spectra with both ethyl isocyanide and phenyl isocyanide dichloride.

Mentioned in this Paper

Metabolic Process, Cellular
Microsomes
Ethyl isocyanide
Cytochrome P450
Hepatic
Cytochrome P-450 Oxygenase
Microsomes, Liver
Mouse, Inbred ICRC
Spectrophotometry, Ultraviolet
Piperonyl Butoxide

About this Paper

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