Spectral resolution of the primary electron acceptor A0 in Photosystem I

The Journal of Physical Chemistry. B
Adrien ChauvetSergei Savikhin

Abstract

The reduced state of the primary electron acceptor of Photosystem I, A(0), was resolved spectroscopically in its lowest energy Q(y) region for the first time without the addition of chemical reducing agents and without extensive data manipulation. To carry this out, we used the menB mutant of Synechocystis sp. PCC 6803 in which phylloquinone is replaced by plastoquinone-9 in the A(1) sites of Photosystem I. The presence of plastoquinone-9 slows electron transfer from A(0) to A(1), leading to a long-lived A(0)(-) state. This allows its spectral signature to be readily detected in a time-resolved optical pump-probe experiment. The maximum bleaching (A(0)(-) - A(0)) was found to occur at 684 nm with a corresponding extinction coefficient of 43 mM(-1) cm(-1). The data show evidence for an electrochromic shift of an accessory chlorophyll pigment, suggesting that the latter Q(y) absorption band is centered around 682 nm.

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Citations

Nov 5, 2014·The Review of Scientific Instruments·Adrien ChauvetMajed Chergui
Jan 14, 2017·Physical Chemistry Chemical Physics : PCCP·Rachna Agarwal, Adrien A P Chauvet
Dec 9, 2014·Physical Chemistry Chemical Physics : PCCP·Adrien A P ChauvetMajed Chergui
Jun 16, 2018·Science·Dennis J NürnbergA William Rutherford
Dec 29, 2019·Biophysical Journal·Dmitry A CherepanovBarry D Bruce
Mar 18, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Dmitry A CherepanovJohn H Golbeck
Feb 27, 2021·Journal of Photochemistry and Photobiology. B, Biology·Dmitry A CherepanovAlexey Yu Semenov
Aug 3, 2021·The Journal of Physical Chemistry Letters·Tomoki KandaHiroshi Ishikita
Sep 4, 2021·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Dmitry A CherepanovAlexey Yu Semenov

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