Spectral sensitivity of single cones in rainbow trout (Oncorhynchus mykiss): a whole-cell voltage clamp study

Vision Research
Leslie G AndersonCraig W Hawryshyn

Abstract

The UVS cone mechanism is known to light adapt at low intensities in comparison to other cones. We were interested in whether this property was related to higher sensitivity in UVS cones or to network adjustments in sensitivity. We investigated spectral sensitivity of 107 individual cone photoreceptors in rainbow trout (Oncorhynchus mykiss) using a whole-cell voltage clamp technique. Mean time-to-peak response was 339+/-90ms and flash sensitivity for a 100ms flash was 4.37x10(-3)+/-2.50x10(-3)pAphotons(-1)microm(2), with no significant differences between the UVS, SWS, MWS and LWS cone classes. The spectral sensitivity of the UVS, SWS and LWS cones conformed to the expression of SWS1, SWS2 and LWS opsin genes. The spectral sensitivity of MWS cones, however, showed clear evidence of co-expression of RH2a and RH2b opsin pigments. The fish used in this study bridged the ontogenetic stage where the MWS cones shift their expression from RH2b to RH2a.

References

Jan 1, 1989·Visual Neuroscience·C W HawryshynP C Martin
Sep 1, 1987·The Journal of Physiology·D A BaylorJ L Schnapf
Jun 1, 1994·Vision Research·C W Hawryshyn, F I Hárosi
Nov 1, 1993·The Journal of Physiology·A G Palacios, T H Goldsmith
Sep 1, 1993·Vision Research·L BeaudetC W Hawryshyn
Jul 1, 1996·The Journal of General Physiology·C L Makino, R L Dodd
Nov 3, 1998·Vision Research·A G PalaciosT H Goldsmith
Dec 30, 1999·The Journal of General Physiology·D A KraaijM Kamermans
Oct 4, 2000·Visual Neuroscience·V I GovardovskiiK Donner
Nov 18, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·C W Hawryshyn
May 15, 2003·The Journal of Comparative Neurology·W Ted AllisonCraig W Hawryshyn
Nov 18, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·S E TempleC W Hawryshyn
Jan 26, 2006·The Journal of Comparative Neurology·Christiana L ChengNorbert H Haunerland
Oct 19, 2006·The Journal of Comparative Neurology·W Ted AllisonCraig W Hawryshyn
Jun 17, 2008·The Journal of Experimental Biology·Shelby E TempleCraig W Hawryshyn
Dec 2, 2008·The Journal of Experimental Biology·S E TempleC W Hawryshyn

❮ Previous
Next ❯

Citations

Jul 10, 2012·Zoology : Analysis of Complex Systems, ZACS·Denis ShcherbakovMartin Blum
Oct 12, 2014·Biology Open·Shyh-Chi ChenCraig W Hawryshyn
Jul 13, 2012·The Journal of Experimental Biology·Shai SabbahCraig W Hawryshyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.