PMID: 45332Apr 1, 1978

Spectral transitions in purple membranes from Halobacterium halobium. I. Effect of preliminary illumination on photochemical processes

Journal of Bioenergetics and Biomembranes
N N Vsevolodov, L N Chekulaeva


The effect of preliminary illumination of purple membranes by yellow light on the difference spectra of short-lived intermediates has been studied. It has been found that changes of the optical density of two of these intermediates, which have the maxima of the difference spectrum at 412 nm and 650 nm, coincides well with the kinetics off the known reversible transitions of the main band of the purple membrane absorption (560 570), i.e., 13-cis-trans transitions. The changes at 412 nm and 650 nm are proportional to the concentration changes of the all-trans retinal and 13-cis retinal, respectively. It was concluded that the formation of the short-lived 412 and 650 intermediates occurs in different photochemical cycles. The pH is found to affect the formation of the 650 intermediate.


May 30, 1975·Biophysics of Structure and Mechanism·N Dencher, M Wilms
Sep 1, 1975·Biophysical Journal·M Chu KungD Oesterhelt
Sep 1, 1975·Biophysical Journal·R H LozierW Stoeckenius
Feb 16, 1976·Biochimica Et Biophysica Acta·W V ShermanS R Caplan
Feb 23, 1976·Biochemical and Biophysical Research Communications·K J KaufmannA Lewis
Jan 1, 1974·Journal of Supramolecular Structure·W Stoeckenius, R H Lozier
Aug 17, 1973·European Journal of Biochemistry·D Oesterhelt, B Hess
Sep 29, 1971·Nature: New Biology·D Oesterhelt, W Stoeckenius

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