Spectral versatility of single reef coral fluorescent proteins detected by spectrally-resolved single molecule spectroscopy

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Christian BlumVinod Subramaniam

Abstract

We use spectrally-resolved room temperature single molecule spectroscopy to yield insights into the occurrence and dynamics of spectral forms of single tetramers of DsRed and its variants DsRed2, Fluorescent Timer, DsRed_N42H and AG4. The red-emitting chromophore in DsRed and all studied variants readily converts into a high quantum efficiency super-red emitting form. We propose the existence of two super-red forms of different quantum efficiencies. The observed emission from the green-emitting chromophore is consistent with bulk spectroscopy. We further observe distinct new spectral forms from each variant, which we attribute to a photoinduced chemical reaction leading to a truncated form of the red-emitting chromophore analogous to the chromophore in the visible fluorescent protein zFP538. Our results have implications for the accurate interpretation of biological and biochemical processes illuminated by fluorescent proteins as well as for choosing appropriate experimental configurations.

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Citations

Oct 16, 2008·Analytical and Bioanalytical Chemistry·Christian Blum, Vinod Subramaniam
Mar 28, 2009·Physical Chemistry Chemical Physics : PCCP·Yanina CesaVinod Subramaniam
Aug 30, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Gregor JungMarc Schneider
Feb 17, 2015·Nanoscale·Laure FabiéThierry Ondarçuhu
Jul 17, 2010·Journal of Biophotonics·Christian BlumVinod Subramaniam
Oct 18, 2011·Beilstein Journal of Nanotechnology·Christian BlumAlfred J Meixner
Jun 6, 2008·The Journal of Physical Chemistry. B·Frank SchleifenbaumAlfred J Meixner
Jan 30, 2013·The Journal of Physical Chemistry. B·Eduard FronVirgile Adam

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