Spectroelectrochemical study of cellobiose dehydrogenase and diaphorase in a thiol-modified gold capillary in the absence of mediators

Bioelectrochemistry
T LarssonTautgirdas Ruzgas

Abstract

A spectroelectrochemical cell was constructed from a gold capillary with 200 microm inner diameter as a working electrode. This allowed spectroelectrochemical study of liquid samples with available volumes less than 5 microl. The optical measurements were accomplished with an optical fibre spectrometer. The optical path of the cell was about 1 cm. To facilitate electrochemistry of biomolecules, the surface of the gold capillary was modified with thiols. The formal potential, E degrees', of the heme in cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium was determined by spectroelectrochemistry in the absence of redox mediators. The number of electrons per redox conversion of heme in CDH was found to be equal to 0.98 + 0.04 corresponding well to a theoretical value representing the redox reaction Fe3+ + e-= Fe2+. Similar spectroelectrochemical experiments with diaphorase from Bacillus stearothermophilus showed the redox conversion of the flavin mononucleotide in diaphorase in the absence of external redox mediators.

References

Mar 1, 1979·Analytical Biochemistry·C W AndersonW R Heineman
Feb 26, 1991·European Journal of Biochemistry·G HenrikssonE Uzcategui
Sep 13, 1993·Biochimica Et Biophysica Acta·G HenrikssonG Pettersson
Mar 22, 2000·Journal of Biotechnology·G HenrikssonG Pettersson
Jun 14, 2000·Analytical Chemistry·A RegisS Servagent-Noinville

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Citations

Jan 27, 2004·Biochemical and Biophysical Research Communications·Nikitas BistolasUlla Wollenberger
Apr 28, 2004·Bioelectrochemistry·Elena E FerapontovaTautgirdas Ruzgas
May 9, 2002·Journal of Biotechnology·Lo Gorton, Elena Domínguez
Apr 19, 2011·Analytical Chemistry·Cynthia A SchrollSamuel A Bryan
Jul 28, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Roland LudwigLo Gorton
Apr 28, 2005·Biosensors & Bioelectronics·Sergey ShleevLo Gorton
Aug 17, 2004·Biosensors & Bioelectronics·Andreas ChristensonTautgirdas Ruzgas
Oct 6, 2006·Biochimica Et Biophysica Acta·Andreas ChristensonLo Gorton
Aug 9, 2008·Angewandte Chemie·Sergey Shleev, Tautgirdas Ruzgas
Apr 25, 2015·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Patrycja KielbInez M Weidinger
Sep 22, 2007·Chemistry & Biology·Miren ZumárragaMiguel Alcalde
Jul 6, 2005·Journal of Biotechnology·L G Vasil'chenkoM L Rabinovich
Sep 9, 2019·Bioelectrochemistry·Stefan Scheiblbrandner, Roland Ludwig
Sep 23, 2006·Bioorganic Chemistry·Sergey ShleevLo Gorton
Apr 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Wolfgang HarreitherRoland Ludwig

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