Spectrofluorimetric determination of oseltamivir phosphate through derivatization with o-phthalaldehyde. Application to pharmaceutical preparations with a preliminary study on spiked plasma samples

Luminescence : the Journal of Biological and Chemical Luminescence
M I WalashM H El-Maghrabey

Abstract

A simple and sensitive spectrofluorimetric method has been developed and validated for the determination of oseltamivir phosphate (OST) in pharmaceutical preparations. The method is based on the reaction between oseltamivir phosphate and o-phthalaldehyde in presence of 2-mercapto-ethanol in borate buffer, pH 10.8, to give a highly fluorescent product measured at 450 nm after excitation at 336 nm. The different experimental parameters affecting the development and stability of the reaction product were studied and optimized. The fluorescence intensity-concentration plot is rectilinear over the range 0.05-1.0 µg/mL, with a lower detection limit of 5 ng/mL and limit of quantitation of 16 ng/mL. The developed method was successfully applied to the analysis of the drug in its commercial capsules and suspension, mean recoveries of OST were 99.97 ± 1.67% and 100.17 ± 1.18%, respectively (n = 3). Statistical comparison of the results obtained by the proposed and comparison method revealed no significant difference in the performance of the two methods regarding accuracy and precision. The proposed method was further extended to in vitro determination of the studied drug in spiked human plasma as a preliminary investigation; the mean re...Continue Reading

References

May 30, 2001·Journal of Pharmaceutical and Biomedical Analysis·A El-GindyM M Shabana
Dec 22, 2006·Journal of Pharmaceutical and Biomedical Analysis·Roman Kand'árZuzana Cervinková
May 22, 2007·Journal of Pharmaceutical and Biomedical Analysis·J Joseph-CharlesJ-P Dubost
Aug 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Makoto YoshitakeMasatoshi Yamaguchi
Mar 4, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gholamreza BahramiAmir Kiani
Apr 9, 2008·Emerging Infectious Diseases·Michael D GreenRobert A Wirtz
Oct 22, 2008·Journal of Pharmaceutical and Biomedical Analysis·Paraskevas D TzanavarasPantelis Rigas
Nov 14, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Katja Heinig, Franz Bucheli
Dec 2, 2008·Chemical & Pharmaceutical Bulletin·Farnaz JabbaribarAbolghasem Jouyban
Jun 23, 2009·Journal of Pharmaceutical and Biomedical Analysis·Evelyne Laborde-KummerJean-Pierre Dubost
Mar 2, 2010·International Journal of Antimicrobial Agents·R DutkowskiB E Davies

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Citations

Nov 19, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Sayed M DerayeaDalia M Nagy

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