Spectrofluorometer: the excitation beam intensity calibration using a single standard solution

Methods and Applications in Fluorescence
Krzysztof Nawara, Jacek Waluk

Abstract

The accurate, periodically updated excitation beam intensity correction is essential for conducting fluorescence spectroscopy research. This article describes a simple and inexpensive approach to reevaluate the excitation calibration curve of a spectrofluorometer using a single dye solution. The method shows excellent agreement with the data obtained using a certified calibration detector for the broad spectral range from 290 nm to 700 nm.

References

Jun 30, 2005·Journal of Fluorescence·J HollandtW Pilz
Jun 30, 2005·Journal of Fluorescence·U Resch-GengerP Nording
Feb 9, 2010·Analytical Chemistry·Paul C DeRose, Ute Resch-Genger
Oct 28, 2016·Chemistry : a European Journal·Jakub OstapkoJacek Waluk
Aug 2, 2017·Analytical Chemistry·Krzysztof Nawara, Jacek Waluk
Mar 26, 2019·Analytical Chemistry·Krzysztof Nawara, Jacek Waluk

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