Spectrofluorometric assay of gentamicin in serum

Clinica Chimica Acta; International Journal of Clinical Chemistry
Z M Habbal

Abstract

A spectrofluorometric method has been developed for the assay of gentamicin in serum. The fluorogen of this reaction is the Zn2+ chelate of N-pyridoxylidene gentamicin. The ratio of gentamicin to Zin is 1.0. Interfering compounds in serum are removed by chromatography on Bio-Rex 70. Recovery of added gentamicin is between 90 and 100%. Determination of gentamicin levels in patient sera correlate well with those done by bioassay. This assay is relatively fast (about 1 h), and requires 1.0 ml of serum. It also can be applied to the determination of other deoxystreptamine antibiotics such as kanamycin and tobramycin.

References

Mar 16, 1972·The New England Journal of Medicine·D H SmithA L Smith

Related Concepts

Gentavet
Hydrogen-Ion Concentration
Fluorescence Spectroscopy
Zinc

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