Spectrofluorometric determination of clonazepam in dosage forms: Application to content uniformity testing and human plasma

Luminescence : the Journal of Biological and Chemical Luminescence
Fathalla F BelalHeba Alaa

Abstract

The present paper describes a developed and validated simple, highly sensitive and cost-effective spectrofluorometric method for determination of clonazepam (CNP). The proposed method depends on forming a highly fluorescent product through the reduction of CNP with Zn/HCl. The produced fluorophore exhibits a strong fluorescence at λem 350 nm after excitation at λex 250 nm. The use of carboxymethylcellulose (CMC) greatly enhanced the fluorescence intensity of the produced fluorophore to the extent of about 100%. Calibration curve showed good linear regression (r2  > 0.9998) within test ranges of 20-400 ng ml-1 with a lower detection limit of 0.67 ng ml-1 and lower quantification limit of 2.22 ng ml-1 upon using CMC. The method was successfully applied to the analysis of CNP in its pharmaceutical formulations and the results were in agreement with those obtained using a reference method. Furthermore, the content uniformity testing of the tablets was also performed. The application of the proposed method was extended to determine CNP in spiked human plasma sample as a preliminary investigation and the results were satisfactory.

References

Nov 10, 2004·Journal of Pharmaceutical and Biomedical Analysis·Isabelle Forfar BaresChristian Jarry
Sep 4, 2015·Luminescence : the Journal of Biological and Chemical Luminescence·Fawzia IbrahimRasha Elsharawy

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Citations

Nov 20, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Soad S Abd El-Hay, Fathalla F Belal
Apr 26, 2020·Journal of Clinical Medicine·Magdalena PaczkowskaJudyta Cielecka-Piontek

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