PMID: 22582646May 16, 2012Paper

Spectrometric characteristics and underlying mechanisms of protective effects of selenium on Spirulina platensis against oxidative stress

Guang pu xue yu guang pu fen xi = Guang pu
Hua-lian WuWenjie Zheng

Abstract

To investigate the possibility and the underlying mechanisms of sodium selenite as antagonist for oxidative stress, the authors examined the effects of pretreatment with selenium on the growth, morphology, spectrometric characteristics and content of reactive oxygen species (ROS) in Spirulina platensis (S. platensis) exposed to H2O2 stress for 24 h in the present study. The results showed that H2O2 induced obvious inhibition of growth and serious morphological damage. The intensity of absorbance peak at 440 nm increased, whereas the peaks at 620 and 680 nm decreased after exposed to H2O2. The emission and excitation spectrum of S. platensis decreased dramatically after H2O2 treatment, and the emission peak from phycocyanin exhibited blue-shift from 660 to 650 nm. The results of FTIR analysis showed that the positions of transmission peaks had no shift, but the relative intensity of characteristic bands from protein and polypeptides including amide I and amide II decreased. Furthermore, the intracellular ROS generation in S. platensis increased significantly in response to H2O2 treatment. In contrast, pretreatments of the cells with selenium for 24 h significantly prevented the H2O2-induced oxidative damages in a dose-dependent ...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.