PMID: 8755Sep 1, 1976

Spectrophotometric characteristics of bilirubin

Pediatric Research
K S Lee, L M Gartner


Spectrophotometric characteristics of bilirubin at low concentrations (0.005-2.500 mg/100 ml) have been studied under various physical conditions in order to gain a better understanding of the state of bilirubin when preparing "solutions" for laboratory use. Standing, minimal shaking, or stirring of the bilirubin preparations at pH 7.4 progressively reduced and altered the maximal spectral absorption of bilirubin (440 nm) in aqueous buffered media. The shift to 415-420 nm is attributed to oxidation of the pigment whereas shoulder formation is attributed to the formation of large size particles (flocculants). In the presence of antixidants (L-ascorbic acid and nitrogen gas) and EDTA the maximal absorption peak remained at 440 nm but decreased in magnitude concomitant with development of progressively increasing shoulder at 480-560 nm. In the absence of antioxidants and EDTA maximal absorption shifted to 415-420 nm and the magnitude of 480-560 nm shoulder formation was less. At the higher concentrations of bilirubin and with reduction in pH of the buffer in the absence of antioxidants, the shift to lower wave lengths was reduced and 450-560 nm shoulder formation was increased. In the absence of antioxidants and EDTA at the lower ...Continue Reading


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