PMID: 20631442Jul 16, 2010Paper

Spectrophotometric determination of trace phosphate ions by amplitude-modulated flow analysis coupled with malachite green method

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Takeshi UemuraHideji Tanaka

Abstract

An amplitude-modulated flow analysis coupled with lock-in detection is proposed for the determination of trace phosphate. The flow rate F(S) of sample solution is varied in response to a periodic signal V(c) of alternating waveform. A coloring reagent solution (ammonium molybdate + Malachite Green) is delivered at a constant flow rate F(R). Under the constant total flow rate F(T), both solutions are merged with a diluent (water). Phosphate ion in the sample reacts with molybdate and then with Malachite Green to form green ion pairs in acidic media. Downstream, the absorbance of the mixed solution is measured at 625 nm. The output voltage V(d) from the detector is sent to a lock-in amplifier, where the wave component of V(d) that has the same frequency as that of V(c) is distinguished from background signals. Phosphate ion can be determined from the amplitude of the component thus extracted. The calibration curve is linear (r(2) > 0.998) and the limit of detection (3.3sigma) is 0.17 micromol dm(-3). Compared with a conventional flow-based method with no modulation, the present method is less susceptible to the baseline drift due mainly to the adsorption of the ion-pair on the optical window, because the quantification is based n...Continue Reading

References

Apr 17, 1999·Science·P H Abelson
Oct 31, 2008·Talanta·José Manuel Estela, Víctor Cerdà
Oct 31, 2008·Talanta·Inês P A MoraisAntónio O S S Rangel

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Citations

Mar 19, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Koji InuiHideji Tanaka
Jul 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yohei KurokawaHideji Tanaka
Sep 15, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yun ZhangMeiqin Hu
Dec 12, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takuya SumitomoHideji Tanaka
Nov 2, 2019·The Analyst·Hongwei ChenQiaoling Du

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