PMID: 8583Aug 1, 1976

Spectrophotometric studies on the pH of frog skeletal muscle. PH change during and after contractile activity

The Journal of General Physiology
V W Macdonald, F F Jöbsis


The spectral characteristics of the pH-sensitive dyes neutral red (NR) and bromcresol purple (BCP) were utilized for studies of the changing intracellular pH (pHi) of sartorius muscles from Rana pipiens, both during the course of an isometric twitch and during recovery metabolism subsequent to a train of twitches. The information from the two dissimilar dyes correlated to confirm the methodology. Neither the fast realkalinization observed during a twitch nor the slow alkalizing phase of recovery metabolism was affected in an obvious manner when phosphocreatine (PC) hydrolysis was blocked by 1-fluoro-2,4-dinitrobenzene (FDNB). Iodoacetic acid (IAA) did inhibit the slow acidic phase of recovery metabolism. The conclusion is made that alkalizing reactions other than PC breakdown must be considered as operative at these levels of activity. Hypertonic solutions altered twitch tension and time course without altering the pHi shifts observed until approximately 75% of the twitch amplitude was abolished. Multiple effects of hypertonic solutions as the muscle approach tonic equilibrium are proposed.


Jan 29, 1971·Science·J Lowenstein, K Tornheim
Dec 23, 1969·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·M J KUSHMERICK, R E DAVIES
Feb 1, 1973·The Journal of General Physiology·J Lännergren, J Noth
Feb 1, 1972·The Journal of Clinical Investigation·S Adler
Oct 12, 1968·Nature·E AprilH Grundfest
Oct 20, 1966·Biochemical and Biophysical Research Communications·F F Jöbsis, M J O'Connor
Aug 7, 1962·Biochemical and Biophysical Research Communications·D F CAIN, R E DAVIES
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Jan 1, 1979·Journal of Neurology·K L Birnberger, M Klepzig
May 15, 1979·Pflügers Archiv : European journal of physiology·S P Robertson, W G Kerrick
Aug 6, 2008·Brain Cell Biology·Bradley J BakerT Knopfel
Apr 1, 1982·The Journal of General Physiology·P Palade, J Vergara
Nov 28, 2006·Journal of Neuroscience Methods·Bradley J BakerE K Kosmidis
Nov 28, 2007·Journal of the International Society of Sports Nutrition·Abbie E SmithJeffrey R Stout
Dec 1, 1977·Archives of Biochemistry and Biophysics·V W MacdonaldF F Jöbsis
Aug 22, 1977·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R MilediG Schalow
Apr 1, 1987·Cell Biophysics·A F Leung, M K Cheung
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Jul 1, 1979·The American Journal of Physiology·S J Hersey

Related Concepts

Bromcresol Purple
Depression, Chemical
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