Abstract
Lignin composition (monolignol types of coniferyl, sinapyl or p-coumaryl alcohol) is causally related to biomass recalcitrance. We describe multiwavelength (220, 228, 240, 250, 260, 290, 295, 300, 310 or 320 nm) absorption spectroscopy of coniferyl alcohol and its laccase- or peroxidase-catalyzed products during real time kinetic, pseudokinetic and endpoint analyses, in optical turn on or turn off modes, under acidic or basic conditions. Reactions in microwell plates and 100 microL volumes demonstrated assay miniaturization and high throughput screening capabilities. Bathochromic and hypsochromic shifts along with hyperchromicity or hypochromicity accompanied enzymatic oxidations by laccase or peroxidase. The limits of detection and quantitation of coniferyl alcohol averaged 2.4 and 7.1 muM respectively, with linear trend lines over 3 to 4 orders of magnitude. Coniferyl alcohol oxidation was evident within 10 minutes or with 0.01 microg/mL laccase and 2 minutes or 0.001 microg/mL peroxidase. Detection limit improved to 1.0 microM coniferyl alcohol with Km of 978.7 +/- 150.7 microM when examined at 260 nm following 30 minutes oxidation with 1.0 microg/mL laccase. Our assays utilized the intrinsic spectroscopic properties of coni...Continue Reading
References
Mar 1, 1992·Journal of Bacteriology·L A CastleR O Morris
Nov 15, 1995·European Journal of Biochemistry·M W FraaijeW J van Berkel
Jun 30, 2001·Phytochemistry·R A Savidge, H Förster
Aug 22, 2001·Journal of Basic Microbiology·A LeonowiczJ Rogalski
Aug 30, 2002·Applied and Environmental Microbiology·Jörg OverhageHorst Priefert
Jan 1, 1996·Plant Physiology·M. M. Campbell, R. R. Sederoff
Feb 1, 1994·Plant Physiology·H. J. MitchellM. S. Barber
Oct 3, 2002·Phytochemistry·Aldwin M Anterola, Norman G Lewis
Dec 17, 2002·Phytochemistry·David FournandCatherine Lapierre
Dec 14, 2004·Comptes rendus biologies·Bernard CathalaRoger Douillard
Jul 20, 2005·Plant Molecular Biology·Sanna KoutaniemiTeemu H Teeri
Dec 22, 2005·Protein Engineering, Design & Selection : PEDS·C MadzakC Jolivalt
Jul 4, 2006·Phytochemistry·Luis L Escamilla-TreviñoJonathan E Poulton
Feb 15, 2007·Journal of Experimental Botany·Bok-Rye LeeTae-Hwan Kim
Apr 11, 2007·The FEBS Journal·Jianfeng JinMarco W Fraaije
Apr 30, 1993·Science·W BaoR R Sederoff
Sep 28, 2007·Combinatorial Chemistry & High Throughput Screening·Komandoor E Achyuthan, David G Whitten
Oct 18, 2008·Analytical Biochemistry·Mohammad-Wadud Bhuiya, Chang-Jun Liu
Oct 24, 2008·Phytopathology·A R De Ascensao, I A Dubery
Oct 31, 2008·Talanta·Anna Jarosz-WilkołazkaLo Gorton
Jan 10, 2009·Genome Biology·Blake A SimmonsHarvey W Blanch
Mar 28, 2009·International Journal of Molecular Sciences·Mohammad J Taherzadeh, Keikhosro Karimi
Jun 18, 2009·Combinatorial Chemistry & High Throughput Screening·Komandoor E AchyuthanDominic Raj
Apr 2, 2010·ACS Applied Materials & Interfaces·Jason C HarperKomandoor E Achyuthan
Mar 1, 2009·Microbial Biotechnology·Francisco J Ruiz-Dueñas, Angel T Martínez