Spectroscopic and biochemical correlations during the course of human lens aging

BMC Ophthalmology
Mala Ranjan, Sashidhar Rao Beedu

Abstract

With age, the human lens accumulates variety of substances that absorbs and fluorescence, which explains the color of yellow, brunescent and nigrescent cataract in terms of aging. The aim of this study was to assess lens fluorophores with properties comparable to those of advanced glycated end products (AGEs) in relation to age in human lenses. These fluorescent compounds are believed to be involved in the development of cataract. Spectroscopic (UV-Vis-NIR) and fluorescence photography (CCD-Digital based image analysis) studies were carried out in randomly selected intact human lenses (2-85 years). AGE-like fluorophores were also measured in water soluble and insoluble (alkali soluble) fractions of human lenses (20-80 years). Our experimental findings suggest that there was a progressive shift in the absorbance characteristic of intact lens in the range of lambda210 nm-lambda470 nm. A relative increase in the absorptivity at lambda(511-520 nm), with age, was also observed. In addition, the ratio of absorptivity at lambda(511-520 nm) versus the maximum absorbance recorded at blue-end cut-off (210-470 nm) was also found to increase, with age. The fluorescent intensity in the intact lens at both UV-B (lambdaEx312 nm) and UV-A (lam...Continue Reading

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Citations

Sep 3, 2014·Analytical Chemistry·Margaret ButkoX Christopher Yu
Apr 11, 2012·Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde·Yoshihito SakanishiKazuhiko Ohnuma
Apr 9, 2009·Journal of Experimental & Clinical Cancer Research : CR·Sebastian Di CesareMiguel N Burnier
Jun 3, 2021·Antibodies·Alexandre Ambrogelly

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BETA
fluorescence studies

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Sigma
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OOI ,
Basic Acquisition Software
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UVI Image Acquisition and Analysis
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