PMID: 36906Jun 5, 1979

Spectroscopic and kinetic properties of the transient intermediate acceptor in reaction centers of Rhodopseudomonas sphaeroides

Biochimica Et Biophysica Acta
Melvin OkamuraG Feher

Abstract

The photoreductive trapping of the transient, intermediate acceptor, I-, in purified reaction centers of Rhodopseudomonas sphaeroides R-26 was investigated for different external conditions. The optical spectrum of I- was found to be similar to that reported for other systems by Shuvalov and Klimov ((1976) Biochim. Biophys. Acta 400, 587--599) and Tiede et al. (P.M. Tiede, R.C. Prince, G.H. Reed and P.L. Dutton (1976) FEBS Lett. 65, 301--304). The optical changes of I- showed characteristics of both bacteriopheophytin (e.g. bleaching at 762, 542 nm and red shift at 400 nm) and bacteriochlorophyll (bleaching at 802 and 590 nm). Two types of EPR signals of I- were observed: one was a narrow singlet at g = 2.0035, deltaH = 13.5 G, the other a doublet with a splitting of 60 G centered around g = 2.00, which was only seen after short illumination times in reaction centers reconstituted with menaquinone. The optical and EPR kinetics of I- on illumination in the presence of reduced cytochrome c and dithionite strongly support the following three-step scheme in which the doublet EPR signal is due to the unstable state DI-Q-Fe2+ and the singlet EPR signal is due to DI-Q2-Fe2+. : formula: (see text), where D is the primary donor (BChl)2+...Continue Reading

References

Sep 1, 1987·Photosynthesis Research·C Kirmaier, D Holten
Nov 1, 1980·Quarterly Reviews of Biophysics·D Devault
May 1, 1984·Quarterly Reviews of Biophysics·A J Hoff
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·V V KlimovB Ke
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Vladimir A Shuvalov, W W Parson
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R F Goldstein, A Bearden
Oct 10, 2013·Photosynthesis Research·Melvin Okamura
Jan 15, 2018·Photosynthesis Research·Hiroki Makita, Gary Hastings
Nov 1, 1981·Quarterly Reviews of Biophysics·A J Hoff
Mar 22, 2012·Physical Chemistry Chemical Physics : PCCP·Aurélien de la LandeDennis R Salahub
Oct 19, 2018·Physical Chemistry Chemical Physics : PCCP·Julian SchäferChristoph Lambert
Nov 19, 2011·Angewandte Chemie·Alexander Heckmann, Christoph Lambert

Citations

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·F J RuzickaR H Sands
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J FajerL D Spaulding
Mar 31, 1975·Biochimica Et Biophysica Acta·W W Parson, R J Cogdell
Jan 11, 1978·Biochimica Et Biophysica Acta·D HoltenJ P Thornber
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M G RockleyW W Parson
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·Melvin OkamuraG Feher
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J J Hopfield

Related Concepts

Ubiquinone (Lab Procedure)
Benzoquinone
Quinones
Darkness
Electron Transport
1,4-benzoquinone
Singlet
Light
Rhodobacter sphaeroides
Spectrophotometry

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