Spectroscopic characterization of recombinant Cu,Zn superoxide dismutase from Photobacterium leiognathi expressed in Escherichia coli: evidence for a novel catalytic copper binding site

Biochemistry
D FotiA Desideri

Abstract

Cu,Zn superoxide dismutase from Photobacterium leiognathi has been cloned and expressed in Escherichia coli. The circular dichroism spectrum in the UV region of the recombinant protein indicates an higher content of random coil structure with respect to the eukaryotic enzymes. Investigation of the active site by optical, CD, and EPR spectroscopy indicates a different coordination geometry around the catalytic copper site with respect to the eukaryotic enzymes. In particular a different orientation of the metal bridging histidine is suggested. The pH dependence of the copper EPR spectrum shows the presence of a single equilibrium which is at least one unit lower than the pK value observed for the bovine enzyme. Despite such structural differences the catalytic rate of this enzyme is identical to that observed for the eukaryotic Cu,Zn superoxide dismutase, suggesting that the overall electric field distribution is similar to that observed in the eukaryotic enzymes.

References

Apr 5, 1990·Journal of Molecular Biology·S M RedfordJ A Tainer
Jun 1, 1985·Journal of Bacteriology·H M Steinman
Sep 15, 1982·Journal of Molecular Biology·J A TainerD C Richardson
May 6, 1994·Journal of Molecular Biology·D BordoM Bolognesi

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Citations

Nov 25, 1998·Protein Science : a Publication of the Protein Society·F PolticelliA Desideri
Dec 3, 1999·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·P Dolashka-AngelovaW Voelter
Mar 18, 1999·Biochemical and Biophysical Research Communications·S FolcarelliA Desideri
Apr 16, 1998·Biochemical and Biophysical Research Communications·S FolcarelliA Desideri
Jun 29, 2000·Biochemical and Biophysical Research Communications·M D'OrazioA Desideri
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R C WadeV Lounnas
May 10, 2012·Free Radical Biology & Medicine·Kalina RanguelovaRonald P Mason
Oct 11, 2005·Biochimica Et Biophysica Acta·Ivana De DomenicoAlessandro Desideri
Sep 10, 1998·Biochemical and Biophysical Research Communications·M E StroppoloA Desideri
Oct 8, 1999·Archives of Biochemistry and Biophysics·F Malvezzi-CampeggiA Desideri
May 21, 1999·Archives of Biochemistry and Biophysics·F VeneriniA Desideri
May 23, 2001·The Journal of Biological Chemistry·F PacelloA Battistoni

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