PMID: 2105744Jan 16, 1990Paper

Spectroscopic characterization of the light-harvesting complex of Rhodospirillum rubrum and its structural subunit

Biochemistry
M C ChangP A Loach

Abstract

The spectroscopic properties of the light-harvesting complex of Rhodospirillum rubrum, B873, and a detergent-isolated subunit form, B820, are presented. Absorption and circular dichroism spectra suggest excitonically interacting bacteriochlorophyll alpha (BChl alpha) molecules give B820 its unique spectroscopic properties. Resonance Raman results indicate that BCHl alpha is 5-coordinate in both B820 and B873 but that the interactions with the BChl C2 acetyl in B820 and B873 are different. The reactivity of BChl alpha in B820 in light and oxygen, or NaBH4, suggests that it is exposed to detergent and the aqueous environment. Excited-state lifetimes of the completely dissociated 777-nm-absorbing form [1.98 ns in 4.5% octyl glucoside (OG)], the intermediate subunit B820 (0.72 ns in 0.8% OG), and the in vivo like reassociated B873 (0.39 ns in 0.3% OG) were measured by single-photon counting. The fluorescence decays were exponential when emission was detected at wavelengths longer than 864 nm. An in vivo like B873 complex, as judged by its spectroscopic properties, can be formed from B820 without the presence of a reaction center.

Citations

Mar 2, 2012·Journal of the American Chemical Society·Joseph W SpringerPaul A Loach
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D OlsenC N Hunter
Dec 1, 1994·Photochemistry and Photobiology·J D Olsen, C N Hunter
Jan 17, 2014·Physical Chemistry Chemical Physics : PCCP·Marco FerrettiRienk van Grondelle
Jul 22, 2014·Photochemistry and Photobiology·Michelle A HarrisJonathan S Lindsey
Jun 15, 2017·The Biochemical Journal·Rafael G Saer, Robert E Blankenship
Sep 3, 2009·The Journal of Physical Chemistry. B·Joanna FiedorLeszek Fiedor

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