Spectroscopic characterization of the NO adduct of hydroxylamine oxidoreductase

Biochemistry
Michael P HendrichAlan B Hooper

Abstract

Hydroxylamine oxidoreductase (HAO) from the autotrophic nitrifying bacterium Nitrosomonas europaea catalyzes the oxidation of NH2OH to NO2-. The enzyme contains eight hemes per subunit which participate in catalysis and electron transport. NO is found to bind to the enzyme and inhibit electron flow to the acceptor protein, cytochrome c554. NO is found to oxidize either partially or fully reduced HAO, but NO will not reduce ferric HAO. Since NO can be reduced but not oxidized to product by HAO, NO is not considered to be a long-lived intermediate in the catalytic mechanism. Substrate oxidation occurs in the presence of bound NO or cyanide, suggesting a second interaction site for substrate with HAO and providing a means for recovery of the NO-inhibited form of the enzyme. Upon addition of NO to oxidized HAO, the integer-spin EPR signal from the active site vanishes, an IR band from NO appears at 1920 cm(-1), and a diamagnetic quadrupole iron doublet appears in Mössbauer spectroscopy with delta = 0.06 mm/s and DeltaEq = 2.1 mm/s. The NO stretching frequency and Mössbauer parameters are characteristic of an [FeNO]6 heme complex. New Mössbauer data on ferric myoglobin-NO are also presented for comparison. The results indicate that ...Continue Reading

References

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Citations

Apr 4, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Bradley O ElmoreAlan B Hooper
Jun 19, 2004·Journal of Bacteriology·Hubertus J E BeaumontRob J M van Spanning
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Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
Oct 4, 2014·The Journal of Physical Chemistry. B·Amr A A Attia, Radu Silaghi-Dumitrescu
Mar 30, 2006·Journal of the American Chemical Society·Anup K UpadhyayMichael P Hendrich
Jun 17, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Joshua KosteraA Andrew Pacheco
Mar 17, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gregory J BodemerA Andrew Pacheco
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Aug 29, 2007·Journal of Inorganic Biochemistry·Damián E BikielDarío A Estrin
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Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D CarantoKyle M Lancaster
May 15, 2009·Chemical Reviews·Victor RoscaMarc T M Koper
Sep 4, 2010·Biochemistry·Joshua KosteraA Andrew Pacheco
Apr 16, 2003·Inorganic Chemistry·Maria Zulema Cabail, A Andrew Pacheco
Feb 13, 2003·Journal of the American Chemical Society·Anup K UpadhyayMichael P Hendrich

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