PMID: 39631Sep 20, 1979

Spectroscopic characterization of the Stentor photoreceptor

Biochimica Et Biophysica Acta
E B WalkerP S Song

Abstract

1. On the basis of chromatographic and spectroscopic (absorption, fluorescence and its polarization, fluorescence lifetime, circular dichroism) characterization of the Stentor photoreceptor (stentorin) for photophobic response, the photoreceptor chromophore released from mild acid hydrolysis has been identified as hypericin. 2. The native chromophore is apparently linked to a protein (65 K) containing Lys and several hydrophobic residues, which is soluble in acetone and n-pentane. The peptide-linked stentorin (I) chromophore exhibits circular dichroism in the visible region due to the induced optical activity provided by the peptide. 3. The sodium dodecyl sulfate polyacrylamide gel electrophoresis of a 38% fraction of the sucrose density centrifugation has resolved stentorin II proteins having molecular weights of 13 000, 16 000, 65 000 and 130 000. These proteins, as well as the acetone-soluble peptide, have been spectroscopically characterized with particular emphasis on their primary photoreactivity as the photophobic receptor of Stentor coeruleus. 4. Irradiation of whole living Stentor in dilute buffer solutions induces a decrease in the pH of the medium. A strong dependence upon pH in the fluorescence spectra of both synth...Continue Reading

References

Mar 1, 1987·Archives of Microbiology·C B HongP S Song
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·K Iwatsuki, P S Song
Jun 1, 1989·Journal of Photochemistry and Photobiology. B, Biology·F LenciP S Song
May 20, 1998·Free Radical Biology & Medicine·S A Johnson, R S Pardini
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·S CarpenterJ W Petrich
Sep 2, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Gáspár Jékely
Mar 29, 2003·Analytical and Bioanalytical Chemistry·Georgios PiperopoulosKlaus-Peter Zeller
Mar 1, 1986·Photochemistry and Photobiology·K C YangM Furuya
Jun 1, 1986·Photochemistry and Photobiology·N Durán, P S Song
Aug 1, 1992·Photochemistry and Photobiology·L N RaserT M Cotton
Sep 1, 1984·Photochemistry and Photobiology·R K PrustiM Häder
Jun 1, 1988·Photochemistry and Photobiology·W Nultsch, D P Häder
Jun 1, 1992·Photochemistry and Photobiology·C Thomas, R S Pardini
Jan 1, 1992·Photochemistry and Photobiology·C ThomasR S Pardini
Jun 1, 1980·Photochemistry and Photobiology·H Shichi, C N Rafferty

Related Concepts

Photoreceptors
Hypericin
Benz(a)Anthracenes
Acetone Measurement
Stentor
Fluorescence Spectroscopy
Centrifugation
Light
Staphylococcal Protein A
Soluble

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