Spectroscopic determination of the thermodynamics of cobalt and zinc binding to GATA proteins

Biochemistry
Amy B GheringHilary A Godwin

Abstract

Vertebrate GATA proteins regulate processes that are vital to development, and each possesses two tandem GATA finger domains: an N-terminal GATA finger and a C-terminal GATA finger. These GATA fingers require Zn(2+) to fold, to bind DNA recognition elements, and to regulate transcription. While the GATA-1 C-terminal finger is necessary and sufficient to bind to single GATA DNA sites, the N-terminal finger interacts with DNA such that the double finger unit (DF domain) has a binding and transactivation profile that is tuned by the DNA-binding site. Co(2+) was used as a spectroscopic probe in a series of competition titrations to determine the affinity of Co(2+) and Zn(2+) for the C-terminal finger from chicken GATA-1 and the double finger from human GATA-1 (referred to in this report as CF and DF). For CF, these experiments yielded K(b)(Co) = 1.0 (+/-1.3) x 10(7) M(-1) and K(b)(Zn) = 2.0 (+/-1.3) x 10(10) M(-1). For DF, these experiments yielded equilibrium constants for the process of two M(2+) binding to form M(2+)(2)-DF of beta(2)(Co) = 2.5 (+/-1.6) x 10(14) M(-2) and beta(2)(Zn) = 6.3 (+/-2.5) x 10(20) M(-2). The ZnS(4) coordination environment of Zn(2+)-bound CF was confirmed with X-ray absorption spectroscopy. A detailed a...Continue Reading

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Citations

Mar 26, 2013·Inorganic Chemistry·Angelique N BesoldSarah L J Michel
Nov 26, 2010·Journal of the American Chemical Society·Olivier Sénèque, Jean-Marc Latour
Jul 4, 2008·Journal of the American Chemical Society·Byoung-Chul LeeRonald N Zuckermann
Jul 30, 2009·Physical Chemistry Chemical Physics : PCCP·Silvia SottiniEdgar J J Groenen
Mar 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Gregor GuncarBostjan Kobe
Jan 22, 2011·Metallomics : Integrated Biometal Science·Susana M QuintalNicholas P Farrell
Sep 29, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Jamie L MichalekSarah L J Michel
Mar 5, 2016·Molecular BioSystems·Angelique N BesoldDavid P Goldberg
Apr 24, 2009·Chemistry : a European Journal·Olivier SénèqueJean-Marc Latour
Dec 23, 2009·Inorganic Chemistry·Dimitrios MaganasEdgar J J Groenen
Jan 8, 2010·Inorganic Chemistry·Seung Jae Lee, Sarah L J Michel
Mar 18, 2005·Journal of the American Chemical Society·Amy B GheringHilary A Godwin
Dec 8, 2004·Inorganic Chemistry·Karen R ThickmanJeremy M Berg
Jun 30, 2005·Journal of the American Chemical Society·John S MagyarHilary Arnold Godwin

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