Spectroscopic labeling of A, S/T in the 1H-15N HSQC spectrum of uniformly (15N-13C) labeled proteins

Journal of Magnetic Resonance
Jeetender Chugh, Ramakrishna V Hosur

Abstract

A new triple resonance two-dimensional experiment, termed (HC)NH, has been described to generate specific labels on the peaks of alanines and serines/threonines, separately, in the (1)H-(15)N HSQC spectrum of a protein. The performance of the pulse sequence has been demonstrated with a 151 residue protein. The method permits the investigation of local environments around those specific residues without actually having to obtain complete resonance assignments for the entire protein. With this one can envisage use of the technique for studying large protein systems from different points of view.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Jan 1, 1995·Journal of Biomolecular NMR·K M LeeJ D Baleja
Apr 1, 2006·Journal of Magnetic Resonance·Amarnath ChatterjeeRamakrishna V Hosur
Dec 29, 2007·Journal of Biomolecular NMR·Jeetender ChughRamakrishna V Hosur

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Citations

Jun 1, 2011·Journal of Biomolecular NMR·Aditi BorkarRamakrishna V Hosur
May 7, 2013·Journal of Biomolecular NMR·Jithender G Reddy, Ramakrishna V Hosur
Mar 20, 2010·Journal of Magnetic Resonance·Dinesh KumarRamakrishna V Hosur

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