PMID: 9659386Jul 11, 1998Paper

Spectroscopic manifestations of flavomononucleotide dimers in polyvinyl alcohol films

Biochimica Et Biophysica Acta
H GrajekC Bojarski

Abstract

Absorption and fluorescence spectra of flavomononucleotide (FMN) in polyvinyl alcohol films (PVA) over a very wide concentration range are investigated. The dimerization constant as well as the pure monomer and dimer spectra are calculated and the structural parameters of FMN dimer are established. Excitation wavelength and temperature dependencies of FMN/PVA fluorescence spectra for different FMN concentrations were carried out. These measurements together with those of absorption reveal that dimers are imperfect traps for excitation energy and that the energy transfer can occur both in forward and in reverse direction. Moreover, it was shown that the observed temperature changes in fluorescence spectra may be qualitatively explained by the effect of inhomogeneous broadening of FMN energy levels and by the presence of fluorescent dimers.

References

Sep 1, 1972·Zeitschrift Für Naturforschung. Teil B. Anorganische Chemie, Organische Chemie, Biochemie, Biophysik, Biologie·P S SongW E Kurtin
Jan 16, 2003·Biochimica Et Biophysica Acta·P BojarskiC Bojarski

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Citations

Jun 7, 2005·Journal of Photochemistry and Photobiology. B, Biology·Hanna GrajekJózef Kuśba
Feb 9, 2005·Biochimica Et Biophysica Acta·B K Muralidhara, Pernilla Wittung-Stafshede
Nov 7, 2006·Journal of Photochemistry and Photobiology. B, Biology·H GrajekG Zurkowska
Jan 16, 2003·Biochimica Et Biophysica Acta·P BojarskiC Bojarski

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