Spectroscopic Photoacoustic Molecular Imaging of Breast Cancer using a B7-H3-targeted ICG Contrast Agent

Theranostics
Katheryne E WilsonJürgen K Willmann

Abstract

Purpose: Breast cancer imaging methods lack diagnostic accuracy, in particular for patients with dense breast tissue, and improved techniques are critically needed. The purpose of this study was to evaluate antibody-indocyanine green (ICG) conjugates, which undergo dynamic absorption spectrum shifts after cellular endocytosis and degradation, and spectroscopic photoacoustic (sPA) imaging to differentiate normal breast tissue from breast cancer by imaging B7-H3, a novel breast cancer associated molecular target. Methods: Quantitative immunohistochemical staining of endothelial and epithelial B7-H3 expression was assessed in 279 human breast tissue samples, including normal (n=53), benign lesions (11 subtypes, n=129), and breast cancers (4 subtypes, n=97). After absorption spectra of intracellular and degraded B7-H3-ICG and Isotype control-ICG (Iso-ICG) were characterized, sPA imaging in a transgenic murine breast cancer model (FVB/N-Tg(MMTVPyMT)634Mul) was performed and compared to imaging of control conditions [B7-H3-ICG in tumor negative animals (n=60), Iso-ICG (n=30), blocking B7-H3+B7-H3-ICG (n=20), and free ICG (n=20)] and validated with ex vivo histological analysis. Results: Immunostaining showed differential B7-H3 expres...Continue Reading

Citations

Jun 13, 2018·Pancreas·Willemieke S TummersRutger-Jan Swijnenburg
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May 2, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Katheryne E WilsonJürgen K Willmann
Feb 13, 2021·NPJ Breast Cancer·Sunitha BachawalKatheryne E Wilson
Apr 10, 2018·Current Opinion in Chemical Biology·Junjie Yao, Lihong V Wang
Oct 4, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Naoki NishioKatheryne E Wilson
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Methods Mentioned

BETA
imaging technique
transgenic
biopsies
affinity purification
gel
flow cytometry
confocal microscopy
fluorescence microscopy
fluorescence imaging

Software Mentioned

FlowJo
Fiji
Prism GraphPad
Living Image
MATLAB

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